{"response":{"status":"ok","message_type":"publication"},"id":101,"citation":"Kawahara et al., 2004, International Journal of Systematic and Evolutionary Microbiology","doi":"10.1099/ijs.0.63260-0","url":"https://api.seqco.de/v1/publications/101.json","link_ext":"https://doi.org/10.1099/ijs.0.63260-0","title":"Ultrastructure and phylogenetic analysis of ‘Candidatus Neoehrlichia mikurensis' in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks","journal":"International Journal of Systematic and Evolutionary Microbiology","journal_loc":"54 (5)","journal_date":"2004-09-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003eA novel bacterium that infects laboratory rats was isolated from wild \u003cjats:italic\u003eRattus norvegicus\u003c/jats:italic\u003e rats in Japan. Transmission electron microscopy of the spleen tissue revealed small cocci surrounded by an inner membrane and a thin, rippled outer membrane in a membrane-bound inclusion within the cytoplasm of endothelial cells. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium found in \u003cjats:italic\u003eR. norvegicus\u003c/jats:italic\u003e rats and \u003cjats:italic\u003eIxodes ovatus\u003c/jats:italic\u003e ticks in Japan revealed that the organism represents a novel clade in the family \u003cjats:italic\u003eAnaplasmataceae\u003c/jats:italic\u003e, which includes the Schotti variant found in \u003cjats:italic\u003eIxodes ricinus\u003c/jats:italic\u003e ticks in the Netherlands and the \u003cjats:italic\u003eEhrlichia\u003c/jats:italic\u003e-like \u003cjats:italic\u003eRattus\u003c/jats:italic\u003e strain found in \u003cjats:italic\u003eR. norvegicus\u003c/jats:italic\u003e rats from China. The novel clade was confirmed by phylogenetic analysis of \u003cjats:italic\u003egroESL\u003c/jats:italic\u003e sequences found in \u003cjats:italic\u003eR. norvegicus\u003c/jats:italic\u003e rats and \u003cjats:italic\u003eIxodes ovatus\u003c/jats:italic\u003e ticks in Japan. No serological cross-reactivity was detected between this bacterium and members of the genera \u003cjats:italic\u003eAnaplasma\u003c/jats:italic\u003e, \u003cjats:italic\u003eEhrlichia\u003c/jats:italic\u003e or \u003cjats:italic\u003eNeorickettsia\u003c/jats:italic\u003e in the family \u003cjats:italic\u003eAnaplasmataceae\u003c/jats:italic\u003e. It is proposed that this new cluster of bacteria should be designated ‘\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Neoehrlichia mikurensis’.\u003c/jats:p\u003e","long_citation_html":"Kawahara et al. (2004). Ultrastructure and phylogenetic analysis of ‘Candidatus Neoehrlichia mikurensis\u0026#39; in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks.\n\u003ci\u003eInternational Journal of Systematic and Evolutionary Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1099/ijs.0.63260-0\" target=\"_blank\"\u003eDOI: 10.1099/ijs.0.63260-0\u003c/a\u003e\n","created_at":"2019-04-15T18:46:05.484Z","updated_at":"2025-11-06T11:25:53.442Z","authors":[{"id":9755,"given":"Makoto","family":"Kawahara","created_at":"2021-12-15T17:22:38.615Z","updated_at":"2021-12-15T17:22:38.615Z","url":"https://api.seqco.de/v1/authors/9755.json"},{"id":2338,"given":"Yasuko","family":"Rikihisa","created_at":"2019-04-15T18:47:17.257Z","updated_at":"2019-04-15T18:47:17.257Z","url":"https://api.seqco.de/v1/authors/2338.json"},{"id":9756,"given":"Emiko","family":"Isogai","created_at":"2021-12-15T17:22:38.641Z","updated_at":"2021-12-15T17:22:38.641Z","url":"https://api.seqco.de/v1/authors/9756.json"},{"id":9757,"given":"Mamoru","family":"Takahashi","created_at":"2021-12-15T17:22:38.655Z","updated_at":"2021-12-15T17:22:38.655Z","url":"https://api.seqco.de/v1/authors/9757.json"},{"id":9758,"given":"Hitoko","family":"Misumi","created_at":"2021-12-15T17:22:38.671Z","updated_at":"2021-12-15T17:22:38.671Z","url":"https://api.seqco.de/v1/authors/9758.json"},{"id":9759,"given":"Chiharu","family":"Suto","created_at":"2021-12-15T17:22:38.686Z","updated_at":"2021-12-15T17:22:38.686Z","url":"https://api.seqco.de/v1/authors/9759.json"},{"id":9760,"given":"Shinichiro","family":"Shibata","created_at":"2021-12-15T17:22:38.706Z","updated_at":"2021-12-15T17:22:38.706Z","url":"https://api.seqco.de/v1/authors/9760.json"},{"id":2337,"given":"Chunbin","family":"Zhang","created_at":"2019-04-15T18:47:17.246Z","updated_at":"2019-04-15T18:47:17.246Z","url":"https://api.seqco.de/v1/authors/2337.json"},{"id":9761,"given":"Masayoshi","family":"Tsuji","created_at":"2021-12-15T17:22:38.749Z","updated_at":"2021-12-15T17:22:38.749Z","url":"https://api.seqco.de/v1/authors/9761.json"}],"names":[{"id":18,"name":"Candidatus Neoehrlichia mikurensis","url":"https://api.seqco.de/v1/names/18.json","uri":"https://seqco.de/i:18"}],"subjects":[{"id":2,"name":"Ecology, Evolution, Behavior and Systematics","url":"https://api.seqco.de/v1/subjects/2.json"},{"id":11,"name":"General Medicine","url":"https://api.seqco.de/v1/subjects/11.json"},{"id":9,"name":"Microbiology","url":"https://api.seqco.de/v1/subjects/9.json"}]}