{"response":{"status":"ok","message_type":"publication"},"id":105,"citation":"Capuzzo et al., 2005, International Journal of Systematic and Evolutionary Microbiology","doi":"10.1099/ijs.0.63653-0","url":"https://api.seqco.de/v1/publications/105.json","link_ext":"https://doi.org/10.1099/ijs.0.63653-0","title":"‘Candidatus Erwinia dacicola’, a coevolved symbiotic bacterium of the olive fly Bactrocera oleae (Gmelin)","journal":"International Journal of Systematic and Evolutionary Microbiology","journal_loc":"55 (4)","journal_date":"2005-07-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003eThe taxonomic identity of the hereditary prokaryotic symbiont of the olive fly\u003cjats:italic\u003eBactrocera oleae\u003c/jats:italic\u003e(Diptera: Tephritidae) was investigated. In order to avoid superficial microbial contaminants and loosely associated saprophytic biota, flies were surface-sterilized at the larval stage and reared under aseptic conditions until adult emergence.\u003cjats:italic\u003eB. oleae\u003c/jats:italic\u003eflies originating from different geographical locations and collected at different times of the year were tested. Bacterial isolation was undertaken from the cephalic oesophageal bulb, which is known to be a specific site of accumulation for the hosted microsymbionts in the adult insect. Despite evidence of multiplication cycles taking place within the insect, attempts at cultivation of the isolated bacteria\u003cjats:italic\u003eex situ\u003c/jats:italic\u003ewere not productive at any stage, leading to the choice of unculturable status definition. PCR amplification and nucleotide sequencing of the entire 16S rRNA gene consistently yielded a single sequence that displayed marked similarity with enterobacterial lineages, with closest matches (97 %) to\u003cjats:italic\u003eErwinia persicina\u003c/jats:italic\u003eand\u003cjats:italic\u003eErwinia rhapontici\u003c/jats:italic\u003e. The novel taxon differs from common intestinal bacterial species of fruit flies and from instances of culturable bacteria previously described in\u003cjats:italic\u003eB. oleae\u003c/jats:italic\u003eraised without sterility precautions, which we also observed as minority occupants or occasional contaminants. The symbiont's identity is also distinct from\u003cjats:italic\u003ePseudomonas savastanoi\u003c/jats:italic\u003e. In all observations, the numerically dominant inhabitant of the olive fly oesophageal organ was the same unculturable organism, whose presence at later stages was also regularly observed in the midgut. A novel species is proposed, by virtue of its unique properties, under the designation ‘\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003eErwinia dacicola’.\u003c/jats:p\u003e","long_citation_html":"Capuzzo et al. (2005). ‘Candidatus Erwinia dacicola’, a coevolved symbiotic bacterium of the olive fly Bactrocera oleae (Gmelin).\n\u003ci\u003eInternational Journal of Systematic and Evolutionary Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1099/ijs.0.63653-0\" target=\"_blank\"\u003eDOI: 10.1099/ijs.0.63653-0\u003c/a\u003e\n","created_at":"2019-04-15T18:46:05.560Z","updated_at":"2025-11-06T11:25:58.489Z","authors":[{"id":9727,"given":"Caterina","family":"Capuzzo","created_at":"2021-12-15T17:22:15.793Z","updated_at":"2021-12-15T17:22:15.793Z","url":"https://api.seqco.de/v1/authors/9727.json"},{"id":2857,"given":"Giuseppe","family":"Firrao","created_at":"2019-04-15T18:47:33.594Z","updated_at":"2019-04-15T18:47:33.594Z","url":"https://api.seqco.de/v1/authors/2857.json"},{"id":9515,"given":"Luca","family":"Mazzon","created_at":"2021-12-15T16:56:52.308Z","updated_at":"2021-12-15T16:56:52.308Z","url":"https://api.seqco.de/v1/authors/9515.json"},{"id":9518,"given":"Andrea","family":"Squartini","created_at":"2021-12-15T16:56:52.355Z","updated_at":"2021-12-15T16:56:52.355Z","url":"https://api.seqco.de/v1/authors/9518.json"},{"id":9519,"given":"Vincenzo","family":"Girolami","created_at":"2021-12-15T16:56:52.369Z","updated_at":"2021-12-15T16:56:52.369Z","url":"https://api.seqco.de/v1/authors/9519.json"}],"names":[{"id":57,"name":"Candidatus Erwinia dacicola","url":"https://api.seqco.de/v1/names/57.json","uri":"https://seqco.de/i:57"}],"subjects":[{"id":2,"name":"Ecology, Evolution, Behavior and Systematics","url":"https://api.seqco.de/v1/subjects/2.json"},{"id":11,"name":"General Medicine","url":"https://api.seqco.de/v1/subjects/11.json"},{"id":9,"name":"Microbiology","url":"https://api.seqco.de/v1/subjects/9.json"}]}