{"response":{"status":"ok","message_type":"publication"},"id":1147,"citation":"Grankvist et al., 2015, Journal of Clinical Microbiology","doi":"10.1128/jcm.00880-15","url":"https://api.seqco.de/v1/publications/1147.json","link_ext":"https://doi.org/10.1128/jcm.00880-15","title":"Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe","journal":"Journal of Clinical Microbiology","journal_loc":"53 (10)","journal_date":"2015-10-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eABSTRACT\u003c/jats:title\u003e\n          \u003cjats:p\u003e\n            “\n            \u003cjats:named-content content-type=\"genus-species\"\u003eCandidatus\u003c/jats:named-content\u003e\n            Neoehrlichia mikurensis” is the tick-borne agent of neoehrlichiosis, an infectious disease that primarily affects immunocompromised patients. So far, the genetic variability of “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Neoehrlichia” has been studied only by comparing 16S rRNA genes and\n            \u003cjats:italic\u003egroEL\u003c/jats:italic\u003e\n            operon sequences. We describe the development and use of a multilocus sequence analysis (MLSA) protocol to characterize the genetic diversity of clinical “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Neoehrlichia” strains in Europe and their relatedness to other species within the\n            \u003cjats:named-content content-type=\"genus-species\"\u003eAnaplasmataceae\u003c/jats:named-content\u003e\n            family. Six genes were selected:\n            \u003cjats:italic\u003eftsZ\u003c/jats:italic\u003e\n            ,\n            \u003cjats:italic\u003eclpB\u003c/jats:italic\u003e\n            ,\n            \u003cjats:italic\u003egatB\u003c/jats:italic\u003e\n            ,\n            \u003cjats:italic\u003elipA\u003c/jats:italic\u003e\n            ,\n            \u003cjats:italic\u003egroEL\u003c/jats:italic\u003e\n            , and 16S rRNA. Each MLSA locus was amplified by real-time PCR, and the PCR products were sequenced. Phylogenetic trees of MLSA locus relatedness were constructed from aligned sequences. Blood samples from 12 patients with confirmed “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Neoehrlichia” infection from Sweden (\n            \u003cjats:italic\u003en\u003c/jats:italic\u003e\n            = 9), the Czech Republic (\n            \u003cjats:italic\u003en\u003c/jats:italic\u003e\n            = 2), and Germany (\n            \u003cjats:italic\u003en\u003c/jats:italic\u003e\n            = 1) were analyzed with the MLSA protocol. Three of the Swedish strains exhibited identical\n            \u003cjats:italic\u003elipA\u003c/jats:italic\u003e\n            sequences, while the\n            \u003cjats:italic\u003elipA\u003c/jats:italic\u003e\n            sequences of the strains from the other nine patients were identical to each other. One of the Czech strains had one differing nucleotide in the\n            \u003cjats:italic\u003eclpB\u003c/jats:italic\u003e\n            sequence from the sequences of the other 11 strains. All 12 strains had identical sequences for the genes 16S rRNA,\n            \u003cjats:italic\u003eftsZ\u003c/jats:italic\u003e\n            ,\n            \u003cjats:italic\u003egatB\u003c/jats:italic\u003e\n            , and\n            \u003cjats:italic\u003egroEL\u003c/jats:italic\u003e\n            . According to the MLSA, among the\n            \u003cjats:named-content content-type=\"genus-species\"\u003eAnaplasmataceae\u003c/jats:named-content\u003e\n            , “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Neoehrlichia” is most closely related to\n            \u003cjats:named-content content-type=\"genus-species\"\u003eEhrlichia ruminantium\u003c/jats:named-content\u003e\n            , less so to\n            \u003cjats:named-content content-type=\"genus-species\"\u003eAnaplasma phagocytophilum\u003c/jats:named-content\u003e\n            , and least to\n            \u003cjats:named-content content-type=\"genus-species\"\u003eWolbachia\u003c/jats:named-content\u003e\n            endosymbionts. To conclude, three sequence types of infectious “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Neoehrlichia” were identified: one in the west of Sweden, one in the Czech Republic, and one spread throughout Europe.\n          \u003c/jats:p\u003e","long_citation_html":"Grankvist et al. (2015). Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe. \n\u003ci\u003eJournal of Clinical Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1128/jcm.00880-15\" target=\"_blank\"\u003eDOI:10.1128/jcm.00880-15\u003c/a\u003e\n","created_at":"2019-04-15T19:26:32.106Z","updated_at":"2025-11-06T13:35:06.208Z","authors":[{"id":4429,"given":"Anna","family":"Grankvist","created_at":"2019-04-15T19:26:32.110Z","updated_at":"2019-04-15T19:26:32.110Z","url":"https://api.seqco.de/v1/authors/4429.json"},{"id":4430,"given":"Edward R. B.","family":"Moore","created_at":"2019-04-15T19:26:32.123Z","updated_at":"2019-04-15T19:26:32.123Z","url":"https://api.seqco.de/v1/authors/4430.json"},{"id":4431,"given":"Liselott","family":"Svensson Stadler","created_at":"2019-04-15T19:26:32.133Z","updated_at":"2019-04-15T19:26:32.133Z","url":"https://api.seqco.de/v1/authors/4431.json"},{"id":3043,"given":"Sona","family":"Pekova","created_at":"2019-04-15T18:47:42.121Z","updated_at":"2019-04-15T18:47:42.121Z","url":"https://api.seqco.de/v1/authors/3043.json"},{"id":4432,"given":"Christian","family":"Bogdan","created_at":"2019-04-15T19:26:32.149Z","updated_at":"2019-04-15T19:26:32.149Z","url":"https://api.seqco.de/v1/authors/4432.json"},{"id":4433,"given":"Walter","family":"Geißdörfer","created_at":"2019-04-15T19:26:32.158Z","updated_at":"2019-04-15T19:26:32.158Z","url":"https://api.seqco.de/v1/authors/4433.json"},{"id":4434,"given":"Jenny","family":"Grip-Lindén","created_at":"2019-04-15T19:26:32.168Z","updated_at":"2019-04-15T19:26:32.168Z","url":"https://api.seqco.de/v1/authors/4434.json"},{"id":4435,"given":"Kenny","family":"Brandström","created_at":"2019-04-15T19:26:32.176Z","updated_at":"2019-04-15T19:26:32.176Z","url":"https://api.seqco.de/v1/authors/4435.json"},{"id":4436,"given":"Jan","family":"Marsal","created_at":"2019-04-15T19:26:32.184Z","updated_at":"2019-04-15T19:26:32.184Z","url":"https://api.seqco.de/v1/authors/4436.json"},{"id":4437,"given":"Kristofer","family":"Andréasson","created_at":"2019-04-15T19:26:32.194Z","updated_at":"2019-04-15T19:26:32.194Z","url":"https://api.seqco.de/v1/authors/4437.json"},{"id":4438,"given":"Catharina","family":"Lewerin","created_at":"2019-04-15T19:26:32.203Z","updated_at":"2019-04-15T19:26:32.203Z","url":"https://api.seqco.de/v1/authors/4438.json"},{"id":4439,"given":"Christina","family":"Welinder-Olsson","created_at":"2019-04-15T19:26:32.213Z","updated_at":"2019-04-15T19:26:32.213Z","url":"https://api.seqco.de/v1/authors/4439.json"},{"id":4440,"given":"Christine","family":"Wennerås","created_at":"2019-04-15T19:26:32.221Z","updated_at":"2019-04-15T19:26:32.221Z","url":"https://api.seqco.de/v1/authors/4440.json"}],"names":[{"id":18,"name":"Candidatus Neoehrlichia mikurensis","url":"https://api.seqco.de/v1/names/18.json","uri":"https://seqco.de/i:18"},{"id":65,"name":"Candidatus Neoehrlichia","url":"https://api.seqco.de/v1/names/65.json","uri":"https://seqco.de/i:65"}],"subjects":[{"id":8,"name":"Microbiology (medical)","url":"https://api.seqco.de/v1/subjects/8.json"}]}