{"response":{"status":"ok","message_type":"publication"},"id":1834,"citation":"Cai et al., 2019, Scientific Reports","doi":"10.1038/s41598-019-55144-4","url":"https://api.seqco.de/v1/publications/1834.json","link_ext":"https://doi.org/10.1038/s41598-019-55144-4","title":"SureSelect targeted enrichment, a new cost effective method for the whole genome sequencing of Candidatus Liberibacter asiaticus","journal":"Scientific Reports","journal_loc":"9 (1)","journal_date":"2019-12-12","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eHuanglongbing (HLB) is a worldwide deadly citrus disease caused by the phloem-limited bacteria ‘\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Liberibacter asiaticus’ (\u003cjats:italic\u003eC\u003c/jats:italic\u003eLas) vectored by Asian citrus psyllids. In order to effectively manage this disease, it is crucial to understand the relationship among the bacterial isolates from different geographical locations. Whole genome sequencing approaches will provide more precise molecular characterization of the diversity among populations. Due to the lack of \u003cjats:italic\u003ein vitro\u003c/jats:italic\u003e culture, obtaining the whole genome sequence of \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas is still a challenge, especially for medium to low titer samples. Hundreds of millions of sequencing reads are needed to get good coverage of \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas from an HLB positive citrus sample. In order to overcome this limitation, we present here a new method, Agilent SureSelect \u003cjats:sup\u003eXT HS\u003c/jats:sup\u003e target enrichment, which can specifically enrich \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas from a metagenomic sample while greatly reducing cost and increasing whole genome coverage of the pathogen. In this study, the \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas genome was successfully sequenced with 99.3% genome coverage and over 72X sequencing coverage from low titer tissue samples (equivalent to 28.52 Cq using Li 16 S qPCR). More importantly, this method also effectively captures regions of diversity in the \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas genome, which provides precise molecular characterization of different strains.\u003c/jats:p\u003e","long_citation_html":"Cai et al. (2019). SureSelect targeted enrichment, a new cost effective method for the whole genome sequencing of Candidatus Liberibacter asiaticus. \n\u003ci\u003eScientific Reports\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1038/s41598-019-55144-4\" target=\"_blank\"\u003eDOI:10.1038/s41598-019-55144-4\u003c/a\u003e\n","created_at":"2019-12-22T07:30:36.502Z","updated_at":"2025-11-06T13:46:06.658Z","authors":[{"id":6849,"given":"Weili","family":"Cai","created_at":"2019-09-29T06:30:52.949Z","updated_at":"2019-09-29T06:30:52.949Z","url":"https://api.seqco.de/v1/authors/6849.json"},{"id":7081,"given":"Schyler","family":"Nunziata","created_at":"2019-12-22T07:30:36.904Z","updated_at":"2019-12-22T07:30:36.904Z","url":"https://api.seqco.de/v1/authors/7081.json"},{"id":3152,"given":"John","family":"Rascoe","created_at":"2019-04-15T18:47:44.611Z","updated_at":"2019-04-15T18:47:44.611Z","url":"https://api.seqco.de/v1/authors/3152.json"},{"id":7082,"given":"Michael J.","family":"Stulberg","created_at":"2019-12-22T07:30:37.238Z","updated_at":"2019-12-22T07:30:37.238Z","url":"https://api.seqco.de/v1/authors/7082.json"}],"names":[{"id":1,"name":"Candidatus Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[{"id":58,"name":"Multidisciplinary","url":"https://api.seqco.de/v1/subjects/58.json"}]}