{"response":{"status":"ok","message_type":"publication"},"id":192,"citation":"Wang et al., 2004, Applied and Environmental Microbiology","doi":"10.1128/aem.70.10.6166-6172.2004","url":"https://api.seqco.de/v1/publications/192.json","link_ext":"https://doi.org/10.1128/aem.70.10.6166-6172.2004","title":"“\u003ci\u003eCandidatus\u003c/i\u003eHepatoplasma crinochetorum,” a New, Stalk-Forming Lineage of\u003ci\u003eMollicutes\u003c/i\u003eColonizing the Midgut Glands of a Terrestrial Isopod","journal":"Applied and Environmental Microbiology","journal_loc":"70 (10)","journal_date":"2004-10-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eABSTRACT\u003c/jats:title\u003e\u003cjats:p\u003eUncultivated bacteria that densely colonize the midgut glands (hepatopancreas) of the terrestrial isopod\u003cjats:italic\u003ePorcellio scaber\u003c/jats:italic\u003e(Crustacea: Isopoda) were identified by cloning and sequencing of their 16S rRNA genes. Phylogenetic analysis revealed that these symbionts represent a novel lineage of the\u003cjats:italic\u003eMollicutes\u003c/jats:italic\u003eand are only distantly related (\u0026lt;82% sequence identity) to members of the\u003cjats:italic\u003eMycoplasmatales\u003c/jats:italic\u003eand\u003cjats:italic\u003eEntomoplasmatales\u003c/jats:italic\u003e. Fluorescence in situ hybridization with a specific oligonucleotide probe confirmed that the amplified 16S rRNA gene sequences indeed originated from a homogeneous population of symbionts intimately associated with the epithelial surface of the hepatopancreas. The same probe also detected morphotypically identical symbionts in other crinochete isopods. Scanning and transmission electron microscopy revealed uniform spherical bacterial cells without a cell wall, sometimes interacting with the microvilli of the brush border by means of stalk-like cytoplasmic appendages, which also appeared to be involved in cell division through budding. Based on the isolated phylogenetic position and unique cytological properties, the provisional name “\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003eHepatoplasma crinochetorum” is proposed for this new taxon of\u003cjats:italic\u003eMollicutes\u003c/jats:italic\u003ecolonizing the hepatopancreas of\u003cjats:italic\u003eP. scaber\u003c/jats:italic\u003e.\u003c/jats:p\u003e","long_citation_html":"Wang et al. (2004). “\u003ci\u003eCandidatus\u003c/i\u003eHepatoplasma crinochetorum,” a New, Stalk-Forming Lineage of\u003ci\u003eMollicutes\u003c/i\u003eColonizing the Midgut Glands of a Terrestrial Isopod. \n\u003ci\u003eApplied and Environmental Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1128/aem.70.10.6166-6172.2004\" target=\"_blank\"\u003eDOI:10.1128/aem.70.10.6166-6172.2004\u003c/a\u003e\n","created_at":"2019-04-15T18:46:17.050Z","updated_at":"2025-11-06T11:27:30.732Z","authors":[{"id":87,"given":"Yongjie","family":"Wang","created_at":"2019-04-15T18:45:42.902Z","updated_at":"2019-04-15T18:45:42.902Z","url":"https://api.seqco.de/v1/authors/87.json"},{"id":88,"given":"Ulrich","family":"Stingl","created_at":"2019-04-15T18:45:42.916Z","updated_at":"2019-04-15T18:45:42.916Z","url":"https://api.seqco.de/v1/authors/88.json"},{"id":84,"given":"Friederike","family":"Anton-Erxleben","created_at":"2019-04-15T18:45:42.869Z","updated_at":"2019-04-15T18:45:42.869Z","url":"https://api.seqco.de/v1/authors/84.json"},{"id":9754,"given":"Sabine","family":"Geisler","created_at":"2021-12-15T17:22:37.014Z","updated_at":"2021-12-15T17:22:37.014Z","url":"https://api.seqco.de/v1/authors/9754.json"},{"id":86,"given":"Andreas","family":"Brune","created_at":"2019-04-15T18:45:42.888Z","updated_at":"2019-04-15T18:45:42.888Z","url":"https://api.seqco.de/v1/authors/86.json"},{"id":85,"given":"Martin","family":"Zimmer","created_at":"2019-04-15T18:45:42.880Z","updated_at":"2019-04-15T18:45:42.880Z","url":"https://api.seqco.de/v1/authors/85.json"}],"names":[{"id":83,"name":"Hepatoplasma crinochetorum","url":"https://api.seqco.de/v1/names/83.json","uri":"https://seqco.de/i:83"},{"id":23995,"name":"Hepatoplasma","url":"https://api.seqco.de/v1/names/23995.json","uri":"https://seqco.de/i:23995"}],"subjects":[{"id":23,"name":"Applied Microbiology and Biotechnology","url":"https://api.seqco.de/v1/subjects/23.json"},{"id":4,"name":"Biotechnology","url":"https://api.seqco.de/v1/subjects/4.json"},{"id":6,"name":"Ecology","url":"https://api.seqco.de/v1/subjects/6.json"},{"id":22,"name":"Food Science","url":"https://api.seqco.de/v1/subjects/22.json"}]}