{"response":{"status":"ok","message_type":"publication"},"id":2310,"citation":"Wang et al., 2021, Nucleic Acids Research","doi":"10.1093/nar/gkab725","url":"https://api.seqco.de/v1/publications/2310.json","link_ext":"https://doi.org/10.1093/nar/gkab725","title":"Sequence specific integration by the family 1 casposase from Candidatus\u003ci\u003eNitrosopumilus koreensis\u003c/i\u003e AR1","journal":"Nucleic Acids Research","journal_loc":"49 (17)","journal_date":"2021-09-27","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eCasposase, a homolog of Cas1 integrase, is encoded by a superfamily of mobile genetic elements known as casposons. While family 2 casposase has been well documented in both function and structure, little is known about the other three casposase families. Here, we studied the family 1 casposase lacking the helix-turn-helix (HTH) domain from Candidatus Nitrosopumilus koreensis AR1 (Ca. N. koreensis). The determinants for integration by Ca. N. koreensis casposase were extensively investigated, and it was found that a 13-bp target site duplication (TSD) sequence, a minimal 3-bp leader and three different nucleotides of the TSD sequences are indispensable for target specific integration. Significantly, the casposase can site-specifically integrate a broad range of terminal inverted repeat (TIR)-derived oligonucleotides ranging from 7-nt to ∼4000-bp, and various oligonucleotides lacking the 5′-TTCTA-3′ motif at the 3′ end of TIR sequence can be integrated efficiently. Furthermore, similar to some Cas1 homologs, the casposase utilizes a 5′-ATAA-3′ motif in the TSD as a molecular ruler to dictate nucleophilic attack at 9-bp downstream of the end of the ruler during the spacer-side integration. By characterizing the family 1 Ca. N. koreensis casposase, we have extended our understanding on mechanistic similarities and evolutionary connections between casposons and the adaptation elements of CRISPR-Cas immunity.\u003c/jats:p\u003e","long_citation_html":"Wang et al. (2021). Sequence specific integration by the family 1 casposase from Candidatus\u003ci\u003eNitrosopumilus koreensis\u003c/i\u003e AR1. \n\u003ci\u003eNucleic Acids Research\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1093/nar/gkab725\" target=\"_blank\"\u003eDOI:10.1093/nar/gkab725\u003c/a\u003e\n","created_at":"2021-08-24T22:00:09.544Z","updated_at":"2025-11-06T13:51:17.237Z","authors":[{"id":8887,"given":"Xiaoke","family":"Wang","created_at":"2021-08-24T22:00:09.567Z","updated_at":"2021-08-24T22:00:09.567Z","url":"https://api.seqco.de/v1/authors/8887.json"},{"id":8888,"given":"Qinling","family":"Yuan","created_at":"2021-08-24T22:00:09.586Z","updated_at":"2021-08-24T22:00:09.586Z","url":"https://api.seqco.de/v1/authors/8888.json"},{"id":8889,"given":"Wenxuan","family":"Zhang","created_at":"2021-08-24T22:00:09.599Z","updated_at":"2021-08-24T22:00:09.599Z","url":"https://api.seqco.de/v1/authors/8889.json"},{"id":8890,"given":"Suyu","family":"Ji","created_at":"2021-08-24T22:00:09.612Z","updated_at":"2021-08-24T22:00:09.612Z","url":"https://api.seqco.de/v1/authors/8890.json"},{"id":8891,"given":"Yang","family":"Lv","created_at":"2021-08-24T22:00:09.625Z","updated_at":"2021-08-24T22:00:09.625Z","url":"https://api.seqco.de/v1/authors/8891.json"},{"id":8892,"given":"Kejing","family":"Ren","created_at":"2021-08-24T22:00:09.637Z","updated_at":"2021-08-24T22:00:09.637Z","url":"https://api.seqco.de/v1/authors/8892.json"},{"id":8893,"given":"Meiling","family":"Lu","created_at":"2021-08-24T22:00:09.651Z","updated_at":"2021-08-24T22:00:09.651Z","url":"https://api.seqco.de/v1/authors/8893.json"},{"id":8894,"given":"Yibei","family":"Xiao","created_at":"2021-08-24T22:00:09.663Z","updated_at":"2021-08-24T22:00:09.663Z","url":"https://api.seqco.de/v1/authors/8894.json"}],"names":[{"id":124,"name":"Candidatus Nitrosopumilus koreensis","url":"https://api.seqco.de/v1/names/124.json","uri":"https://seqco.de/i:124"}],"subjects":[{"id":15,"name":"Genetics","url":"https://api.seqco.de/v1/subjects/15.json"}]}