{"response":{"status":"ok","message_type":"publication"},"id":3055,"citation":"Wang et al., 2023, Molecular Horticulture","doi":"10.1186/s43897-023-00062-3","url":"https://api.seqco.de/v1/publications/3055.json","link_ext":"https://doi.org/10.1186/s43897-023-00062-3","title":"Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus","journal":"Molecular Horticulture","journal_loc":"3 (1)","journal_date":"2023-07-24","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eCitrus Huanglongbing (HLB), caused by \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Liberibacter asiaticus (\u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas), is the most serious disease worldwide. \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460 was previously characterized as a potential virulence factor of \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas. However, the function and mechanism of \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460 involved in \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas against citrus is still elusive. Here, we showed that transgenic expression of \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460 in Wanjincheng oranges (\u003cjats:italic\u003eC. sinensis\u003c/jats:italic\u003e Osbeck) contributed to the early growth of \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas and the development of symptoms. When the temperature increased from 25 °C to 32 °C, \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas growth and symptom development in transgenic plants were slower than those in WT controls. RNA-seq analysis of transgenic plants showed that \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460 affected multiple biological processes. At 25 °C, transcription activities of the “Protein processing in endoplasmic reticulum” and “Cyanoamino acid metabolism” pathways increased while transcription activities of many pathways decreased at 32 °C. 124 and 53 genes, separately annotated to plant-pathogen interaction and MAPK signaling pathways, showed decreased expression at 32 °C, compared with these (38 for plant-pathogen interaction and 17 for MAPK signaling) at 25 °C. Several important genes (MAPKKK14, HSP70b, NCED3 and WRKY33), remarkably affected by \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460, were identified. Totally, our data suggested that \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLasSDE460 participated in the pathogenesis of \u003cjats:italic\u003eCa\u003c/jats:italic\u003eLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent.\u003c/jats:p\u003e\n                \u003cjats:p\u003e\u003cjats:bold\u003eGraphical Abstract\u003c/jats:bold\u003e\u003c/jats:p\u003e","long_citation_html":"Wang et al. (2023). Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus.\n\u003ci\u003eMolecular Horticulture\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1186/s43897-023-00062-3\" target=\"_blank\"\u003eDOI: 10.1186/s43897-023-00062-3\u003c/a\u003e\n","created_at":"2023-07-25T22:00:16.280Z","updated_at":"2025-11-06T14:16:53.430Z","authors":[{"id":9951,"given":"Shuai","family":"Wang","created_at":"2022-02-21T23:00:10.217Z","updated_at":"2022-02-21T23:00:10.217Z","url":"https://api.seqco.de/v1/authors/9951.json"},{"id":9950,"given":"Meixia","family":"Du","created_at":"2022-02-21T23:00:10.206Z","updated_at":"2022-02-21T23:00:10.206Z","url":"https://api.seqco.de/v1/authors/9950.json"},{"id":9952,"given":"Liting","family":"Dong","created_at":"2022-02-21T23:00:10.228Z","updated_at":"2022-02-21T23:00:10.228Z","url":"https://api.seqco.de/v1/authors/9952.json"},{"id":9953,"given":"Rongrong","family":"Qu","created_at":"2022-02-21T23:00:10.239Z","updated_at":"2022-02-21T23:00:10.239Z","url":"https://api.seqco.de/v1/authors/9953.json"},{"id":12115,"given":"Danlu","family":"Ran","created_at":"2023-07-25T22:00:16.359Z","updated_at":"2023-07-25T22:00:16.359Z","url":"https://api.seqco.de/v1/authors/12115.json"},{"id":12116,"given":"Juanjuan","family":"Ma","created_at":"2023-07-25T22:00:16.370Z","updated_at":"2023-07-25T22:00:16.370Z","url":"https://api.seqco.de/v1/authors/12116.json"},{"id":452,"given":"Xuefeng","family":"Wang","created_at":"2019-04-15T18:46:07.997Z","updated_at":"2019-04-15T18:46:07.997Z","url":"https://api.seqco.de/v1/authors/452.json"},{"id":12117,"given":"Lanzhen","family":"Xu","created_at":"2023-07-25T22:00:16.388Z","updated_at":"2023-07-25T22:00:16.388Z","url":"https://api.seqco.de/v1/authors/12117.json"},{"id":6637,"given":"Weimin","family":"Li","created_at":"2019-07-28T06:31:04.969Z","updated_at":"2019-07-28T06:31:04.969Z","url":"https://api.seqco.de/v1/authors/6637.json"},{"id":9955,"given":"Yongrui","family":"He","created_at":"2022-02-21T23:00:10.264Z","updated_at":"2022-02-21T23:00:10.264Z","url":"https://api.seqco.de/v1/authors/9955.json"},{"id":8231,"given":"Xiuping","family":"Zou","created_at":"2021-03-18T08:44:14.728Z","updated_at":"2021-03-18T08:44:14.728Z","url":"https://api.seqco.de/v1/authors/8231.json"}],"names":[{"id":1,"name":"Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[{"id":62,"name":"Earth-Surface Processes","url":"https://api.seqco.de/v1/subjects/62.json"}]}