{"response":{"status":"ok","message_type":"publication"},"id":3169,"citation":"Kuroda et al., 2023, ","doi":"10.1101/2023.08.25.554742","url":"https://api.seqco.de/v1/publications/3169.json","link_ext":"https://doi.org/10.1101/2023.08.25.554742","title":"Microscopic and metatranscriptomic analyses revealed unique cross-domain symbiosis between\u003ci\u003eCandidatus\u003c/i\u003ePatescibacteria/candidate phyla radiation (CPR) and methanogenic archaea in anaerobic ecosystems","journal":"","journal_loc":"","journal_date":"2023-08-25","pub_type":"posted-content","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eTo verify the parasitic lifestyle of\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003ePatescibacteria in the enrichment cultures derived from a methanogenic bioreactor, we applied multifaceted approaches combining cultivation, microscopy, metatranscriptomic, and protein structure prediction analyses. Cultivation experiments with the addition of exogenous methanogenic archaea with acetate, amino acids, and nucleoside monophosphates and 16S rRNA gene sequencing confirmed the increase in the relative abundance of\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Patescibacteria and methanogens. The predominant\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Patescibacteria were\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Yanofskybacteria and 32-520 lineages (to which belongs to class\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Paceibacteria) and positive linear relationships (\u003cjats:italic\u003er\u003c/jats:italic\u003e\u003cjats:sup\u003e\u003cjats:italic\u003e2\u003c/jats:italic\u003e\u003c/jats:sup\u003e≥ 0.70) between the relative abundance of\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Yanofskybacteria and\u003cjats:italic\u003eMethanothrix\u003c/jats:italic\u003e, suggesting that the tendency of the growth rate is similar to that of the host. By fluorescence\u003cjats:italic\u003ein situ\u003c/jats:italic\u003ehybridization (FISH) observations, the FISH signals of\u003cjats:italic\u003eMethanothrix\u003c/jats:italic\u003eand\u003cjats:italic\u003eMethanospirillum\u003c/jats:italic\u003ecells with\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Yanofskybacteria and with 32-520 lineages, respectively, were significantly lower than those of the methanogens without\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Patescibacteria, suggesting their parasitic interaction. The TEM and SEM observations also support parasitism in that the cell walls and plugs of these methanogens associated with submicron cells were often deformed. In particular, some\u003cjats:italic\u003eMethanothrix\u003c/jats:italic\u003e-like filamentous cells were dented where the submicron cells were attached. Metatranscriptomic and protein structure prediction analyses identified highly expressed secreted genes from the genomes of\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Yanofskybacteria and 32-520, and these genes contain adhesion-related domains to the host cells. Considering the results through the combination of microscopic observations, gene expression, and computational protein modeling, we propose that the interactions between\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Yanofskybacteria and 32-520 belonging to class\u003cjats:italic\u003eCa\u003c/jats:italic\u003e. Paceibacteria and methanogenic archaea are parasitism.\u003c/jats:p\u003e","long_citation_html":"Kuroda et al. (2023). Microscopic and metatranscriptomic analyses revealed unique cross-domain symbiosis between\u003ci\u003eCandidatus\u003c/i\u003ePatescibacteria/candidate phyla radiation (CPR) and methanogenic archaea in anaerobic ecosystems. \n\u003ci\u003e\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1101/2023.08.25.554742\" target=\"_blank\"\u003eDOI:10.1101/2023.08.25.554742\u003c/a\u003e\n","created_at":"2023-08-27T22:00:16.601Z","updated_at":"2025-11-06T14:18:05.398Z","authors":[{"id":8770,"given":"Kyohei","family":"Kuroda","created_at":"2021-07-23T18:12:28.908Z","updated_at":"2021-07-23T18:12:28.908Z","url":"https://api.seqco.de/v1/authors/8770.json"},{"id":12614,"given":"Meri","family":"Nakajima","created_at":"2023-08-27T22:00:16.631Z","updated_at":"2023-08-27T22:00:16.631Z","url":"https://api.seqco.de/v1/authors/12614.json"},{"id":8722,"given":"Ryosuke","family":"Nakai","created_at":"2021-07-12T11:58:02.035Z","updated_at":"2021-07-12T11:58:02.035Z","url":"https://api.seqco.de/v1/authors/8722.json"},{"id":9940,"given":"Yuga","family":"Hirakata","created_at":"2022-02-17T23:00:11.967Z","updated_at":"2022-02-17T23:00:11.967Z","url":"https://api.seqco.de/v1/authors/9940.json"},{"id":12615,"given":"Shuka","family":"Kagemasa","created_at":"2023-08-27T22:00:16.653Z","updated_at":"2023-08-27T22:00:16.653Z","url":"https://api.seqco.de/v1/authors/12615.json"},{"id":1860,"given":"Kengo","family":"Kubota","created_at":"2019-04-15T18:46:56.930Z","updated_at":"2019-04-15T18:46:56.930Z","url":"https://api.seqco.de/v1/authors/1860.json"},{"id":12616,"given":"Taro Q.P.","family":"Noguchi","created_at":"2023-08-27T22:00:16.666Z","updated_at":"2023-08-27T22:00:16.666Z","url":"https://api.seqco.de/v1/authors/12616.json"},{"id":10683,"given":"Kyosuke","family":"Yamamoto","created_at":"2022-08-31T22:00:22.491Z","updated_at":"2022-08-31T22:00:22.491Z","url":"https://api.seqco.de/v1/authors/10683.json"},{"id":1932,"given":"Hisashi","family":"Satoh","created_at":"2019-04-15T18:47:02.453Z","updated_at":"2019-04-15T18:47:02.453Z","url":"https://api.seqco.de/v1/authors/1932.json"},{"id":1859,"given":"Masaru K.","family":"Nobu","created_at":"2019-04-15T18:46:56.916Z","updated_at":"2019-04-15T18:46:56.916Z","url":"https://api.seqco.de/v1/authors/1859.json"},{"id":8769,"given":"Takashi","family":"Narihiro","created_at":"2021-07-23T18:12:28.884Z","updated_at":"2021-07-23T18:12:28.884Z","url":"https://api.seqco.de/v1/authors/8769.json"}],"names":[{"id":32305,"name":"Paceibacteria","url":"https://api.seqco.de/v1/names/32305.json","uri":"https://seqco.de/i:32305"},{"id":32307,"name":"Yanofskyibacteriota","url":"https://api.seqco.de/v1/names/32307.json","uri":"https://seqco.de/i:32307"}],"subjects":[]}