{"response":{"status":"ok","message_type":"publication"},"id":3235,"citation":"Horn et al., 1999, Environmental Microbiology","doi":"10.1046/j.1462-2920.1999.00045.x","url":"https://api.seqco.de/v1/publications/3235.json","link_ext":"https://doi.org/10.1046/j.1462-2920.1999.00045.x","title":"Novel bacterial endosymbionts of\n                    \u003ci\u003eAcanthamoeba\u003c/i\u003e\n                    spp. related to the\n                    \u003ci\u003eParamecium caudatum\u003c/i\u003e\n                    symbiont\n                    \u003ci\u003eCaedibacter caryophilus\u003c/i\u003e","journal":"Environmental Microbiology","journal_loc":"1 (4)","journal_date":"1999-08-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003e\n                    Acanthamoebae are increasingly being recognized as hosts for obligate bacterial endosymbionts, most of which are presently uncharacterized. In this study, the phylogeny of three Gram‐negative, rod‐shaped endosymbionts and their\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    host cells was analysed by the rRNA approach. Comparative analyses of 16S rDNA sequences retrieved from amoebic cell lysates revealed that the endosymbionts of\n                    \u003cjats:italic\u003eAcanthamoeba polyphaga\u003c/jats:italic\u003e\n                    HN‐3,\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. UWC9 and\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. UWE39 are related to the\n                    \u003cjats:italic\u003eParamecium caudatum\u003c/jats:italic\u003e\n                    endosymbionts\n                    \u003cjats:italic\u003eCaedibacter caryophilus, Holospora elegans a\u003c/jats:italic\u003e\n                    n\n                    \u003cjats:italic\u003ed Holospora obtusa\u003c/jats:italic\u003e\n                    . With overall 16S rRNA sequence similarities to their closest relative,\n                    \u003cjats:italic\u003eC. caryophilus\u003c/jats:italic\u003e\n                    , of between 87% and 93%, these endosymbionts represent three distinct new species.\n                    \u003cjats:italic\u003eIn situ\u003c/jats:italic\u003e\n                    hybridization with fluorescently labelled endosymbiont‐specific 16S rRNA‐targeted probes demonstrated that the retrieved 16S rDNA sequences originated from the endosymbionts and confirmed their intracellular localization. We propose to classify provisionally the endosymbiont of\n                    \u003cjats:italic\u003eAcanthamoeba polyphaga\u003c/jats:italic\u003e\n                    HN‐3 as ‘\n                    \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e\n                    Caedibacter acanthamoebae’, the endosymbiont of\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. strain UWC9 as ‘\n                    \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e\n                    Paracaedibacter acanthamoebae’ and the endosymbiont of\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. strain UWE39 as ‘\n                    \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e\n                    Paracaedibacter symbiosus’. The phylogeny of the\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    host cells was analysed by comparative sequence analyses of their 18S rRNA. Although\n                    \u003cjats:italic\u003eAcanthamoeba polyphaga\u003c/jats:italic\u003e\n                    HN‐3 clearly groups together with most of the known\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    isolates (18S rRNA sequence type 4),\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. UWC9 and UWE39 exhibit \u0026lt; 92% 18S rRNA sequence similarity to each other and to other\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    isolates. Therefore, we propose two new sequence types (T13 and T14) within the genus\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    containing, respectively,\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. UWC9 and\n                    \u003cjats:italic\u003eAcanthamoeba\u003c/jats:italic\u003e\n                    sp. UWE39.\n                  \u003c/jats:p\u003e","long_citation_html":"Horn et al. (1999). Novel bacterial endosymbionts of\n                    \u003ci\u003eAcanthamoeba\u003c/i\u003e\n                    spp. related to the\n                    \u003ci\u003eParamecium caudatum\u003c/i\u003e\n                    symbiont\n                    \u003ci\u003eCaedibacter caryophilus\u003c/i\u003e. \n\u003ci\u003eEnvironmental Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1046/j.1462-2920.1999.00045.x\" target=\"_blank\"\u003eDOI:10.1046/j.1462-2920.1999.00045.x\u003c/a\u003e\n","created_at":"2023-10-20T09:24:22.838Z","updated_at":"2025-11-06T14:18:49.028Z","authors":[{"id":321,"given":"Matthias","family":"Horn","created_at":"2019-04-15T18:46:01.805Z","updated_at":"2019-04-15T18:46:01.805Z","url":"https://api.seqco.de/v1/authors/321.json"},{"id":9720,"given":"Thomas R.","family":"Fritsche","created_at":"2021-12-15T17:22:05.259Z","updated_at":"2021-12-15T17:22:05.259Z","url":"https://api.seqco.de/v1/authors/9720.json"},{"id":12864,"given":"Romesh K.","family":"Gautom","created_at":"2023-10-20T09:24:22.927Z","updated_at":"2023-10-20T09:24:22.927Z","url":"https://api.seqco.de/v1/authors/12864.json"},{"id":9339,"given":"Karl‐Heinz","family":"Schleifer","created_at":"2021-12-15T16:42:36.568Z","updated_at":"2021-12-15T16:42:36.568Z","url":"https://api.seqco.de/v1/authors/9339.json"},{"id":324,"given":"Michael","family":"Wagner","created_at":"2019-04-15T18:46:01.846Z","updated_at":"2019-04-15T18:46:01.846Z","url":"https://api.seqco.de/v1/authors/324.json"}],"names":[{"id":32875,"name":"Paracaedimonas acanthamoebae","url":"https://api.seqco.de/v1/names/32875.json","uri":"https://seqco.de/i:32875"}],"subjects":[{"id":2,"name":"Ecology, Evolution, Behavior and Systematics","url":"https://api.seqco.de/v1/subjects/2.json"},{"id":9,"name":"Microbiology","url":"https://api.seqco.de/v1/subjects/9.json"}]}