{"response":{"status":"ok","message_type":"publication"},"id":3286,"citation":"Nian et al., 2024, Molecular Ecology","doi":"10.1111/mec.17214","url":"https://api.seqco.de/v1/publications/3286.json","link_ext":"https://doi.org/10.1111/mec.17214","title":"Infection with ‘\u003ci\u003eCandidatus\u003c/i\u003e Liberibacter asiaticus’ improves the fecundity of \u003ci\u003eDiaphorina citri\u003c/i\u003e aiding its proliferation: A win‐win strategy","journal":"Molecular Ecology","journal_loc":"33 (2)","journal_date":"2024-01-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eThe evolution of insect vector‐pathogen relationships has long been of interest in the field of molecular ecology. One system of special relevance, due to its economic impacts, is that between \u003cjats:italic\u003eDiaphorina citri\u003c/jats:italic\u003e and ‘\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Liberibacter asiaticus’ (\u003cjats:italic\u003eC\u003c/jats:italic\u003eLas), the cause of the severe Asian form of huanglongbing. \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas‐positive \u003cjats:italic\u003eD. citri\u003c/jats:italic\u003e are more fecund than their \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas‐negative counterparts, boosting opportunities for pathogens to acquire new vector hosts. The molecular mechanism behind this life‐history shift remains unclear. Here, we found that \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas promoted ovarian development and increased the expression of the \u003cjats:italic\u003evitellogenin receptor\u003c/jats:italic\u003e (\u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e) in ovaries. \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e RNAi significantly decreased fecundity and \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas titer in ovaries, extended the preoviposition period, shortened the oviposition period and blocked ovarian development. Given their importance in gene regulation, we explored the role of miRNAs in shaping these phenotypes and their molecular triggers. Our results showed that one miRNA, miR‐275, suppressed \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e expression by binding to its 3' UTR. Overexpression of miR‐275 knocked down \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e expression and \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas titer in ovaries, causing reproductive defects that mimicked \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e knockdown phenotypes. We focused, further, on roles of the Juvenile Hormone (JH) pathway in shaping the observed fecundity phenotype, given its known impacts on ovarian development. After \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas infection, this pathway was upregulated, thereby increasing \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e expression. From these combined results, we conclude that \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas hijacks the JH signalling pathway and miR‐275, thereby targeting \u003cjats:italic\u003eDcVgR\u003c/jats:italic\u003e to increase \u003cjats:italic\u003eD. citri\u003c/jats:italic\u003e fecundity. These changes simultaneously increase \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas replication, suggesting a pathogen‐vector host mutualism, or a seemingly helpful, but cryptically costly life‐history manipulation.\u003c/jats:p\u003e","long_citation_html":"Nian et al. (2024). Infection with ‘\u003ci\u003eCandidatus\u003c/i\u003e Liberibacter asiaticus’ improves the fecundity of \u003ci\u003eDiaphorina citri\u003c/i\u003e aiding its proliferation: A win‐win strategy. \n\u003ci\u003eMolecular Ecology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1111/mec.17214\" target=\"_blank\"\u003eDOI:10.1111/mec.17214\u003c/a\u003e\n","created_at":"2023-11-30T23:00:49.114Z","updated_at":"2025-11-06T14:19:25.522Z","authors":[{"id":11653,"given":"Xiaoge","family":"Nian","created_at":"2023-05-20T22:00:17.208Z","updated_at":"2023-05-20T22:00:17.208Z","url":"https://api.seqco.de/v1/authors/11653.json"},{"id":11656,"given":"Yaru","family":"Luo","created_at":"2023-05-20T22:00:17.232Z","updated_at":"2023-05-20T22:00:17.232Z","url":"https://api.seqco.de/v1/authors/11656.json"},{"id":13088,"given":"Xinyu","family":"He","created_at":"2023-11-30T23:00:49.184Z","updated_at":"2023-11-30T23:00:49.184Z","url":"https://api.seqco.de/v1/authors/13088.json"},{"id":11654,"given":"Shujie","family":"Wu","created_at":"2023-05-20T22:00:17.216Z","updated_at":"2023-05-20T22:00:17.216Z","url":"https://api.seqco.de/v1/authors/11654.json"},{"id":11655,"given":"Jiayun","family":"Li","created_at":"2023-05-20T22:00:17.224Z","updated_at":"2023-05-20T22:00:17.224Z","url":"https://api.seqco.de/v1/authors/11655.json"},{"id":11659,"given":"Desen","family":"Wang","created_at":"2023-05-20T22:00:17.255Z","updated_at":"2023-05-20T22:00:17.255Z","url":"https://api.seqco.de/v1/authors/11659.json"},{"id":5570,"given":"Paul","family":"Holford","created_at":"2019-04-15T19:29:33.367Z","updated_at":"2019-04-15T19:29:33.367Z","url":"https://api.seqco.de/v1/authors/5570.json"},{"id":6701,"given":"George Andrew Charles","family":"Beattie","created_at":"2019-08-25T06:31:04.162Z","updated_at":"2019-08-25T06:31:04.162Z","url":"https://api.seqco.de/v1/authors/6701.json"},{"id":5454,"given":"Yijing","family":"Cen","created_at":"2019-04-15T19:29:30.542Z","updated_at":"2019-04-15T19:29:30.542Z","url":"https://api.seqco.de/v1/authors/5454.json"},{"id":13089,"given":"Songdou","family":"Zhang","created_at":"2023-11-30T23:00:49.235Z","updated_at":"2023-11-30T23:00:49.235Z","url":"https://api.seqco.de/v1/authors/13089.json"},{"id":5571,"given":"Yurong","family":"He","created_at":"2019-04-15T19:29:33.376Z","updated_at":"2019-04-15T19:29:33.376Z","url":"https://api.seqco.de/v1/authors/5571.json"}],"names":[{"id":1,"name":"Candidatus Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[{"id":2,"name":"Ecology, Evolution, Behavior and Systematics","url":"https://api.seqco.de/v1/subjects/2.json"},{"id":15,"name":"Genetics","url":"https://api.seqco.de/v1/subjects/15.json"}]}