{"response":{"status":"ok","message_type":"publication"},"id":3335,"citation":"Lee et al., 2024, ","doi":"10.1101/2024.01.08.574571","url":"https://api.seqco.de/v1/publications/3335.json","link_ext":"https://doi.org/10.1101/2024.01.08.574571","title":"“\u003ci\u003eCa\u003c/i\u003e. Nitrosocosmicus” members are the dominant archaea associated with pepper (\u003ci\u003eCapsicum annuum\u003c/i\u003eL.) and ginseng (\u003ci\u003ePanax ginseng\u003c/i\u003eC.A. Mey.) plants’ rhizospheres","journal":"","journal_loc":"","journal_date":"2024-01-08","pub_type":"posted-content","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:sec\u003e\u003cjats:title\u003eBackground\u003c/jats:title\u003e\u003cjats:p\u003eAlthough archaea are widespread in terrestrial environments, little is known about the selection forces that shape their composition, functions, survival, and proliferation strategies in the rhizosphere. The ammonia-oxidizing archaea (AOA), which are abundant in soil environments, catalyze the first step of nitrification and have the potential to influence plant growth and development significantly.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eResults\u003c/jats:title\u003e\u003cjats:p\u003eBased on archaeal 16S rRNA and\u003cjats:italic\u003eamoA\u003c/jats:italic\u003egene (encoding the ammonia monooxygenase subunit A) amplicon sequencing analysis, distinct archaeal communities dominated by AOA were found to be associated with the root systems of pepper (\u003cjats:italic\u003eCapsicum annuum\u003c/jats:italic\u003eL.) and ginseng (\u003cjats:italic\u003ePanax ginseng\u003c/jats:italic\u003eC.A. Mey.) plants compared to bulk soil not penetrated by roots. AOA related to “\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003eNitrosocosmicus”, which, unlike most other AOA, harbor genes encoding manganese catalase (MnKat), dominated rhizosphere soils, and thus contributed to the development of distinct archaeal communities in rhizospheres. Accordingly, for both plant species, the copy number ratios of AOA MnKat genes to\u003cjats:italic\u003eamoA\u003c/jats:italic\u003egenes were significantly higher in rhizosphere soils than in bulk soils. In contrast to MnKat-negative strains from other AOA clades, the catalase activity of a representative isolate of “\u003cjats:italic\u003eCa.\u003c/jats:italic\u003eNitrosocosmicus” was demonstrated. Members of this clade were enriched in H\u003cjats:sub\u003e2\u003c/jats:sub\u003eO\u003cjats:sub\u003e2\u003c/jats:sub\u003e-amended bulk soils, and constitutive expression of their MnKat gene was observed in both bulk and rhizosphere soils.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eConclusions\u003c/jats:title\u003e\u003cjats:p\u003eDue to their abundance, “\u003cjats:italic\u003eCa.\u003c/jats:italic\u003eNitrosocosmicus” members can be considered key players mediating the nitrification process in rhizospheres. The selection of this MnKat-containing AOA in rhizospheres of several agriculturally important plants hints at a previously overlooked AOA-plant interaction. For additional mechanistic analyses of the interaction, this key clade of AOA with cultured representatives can be employed.\u003c/jats:p\u003e\u003c/jats:sec\u003e","long_citation_html":"Lee et al. (2024). “\u003ci\u003eCa\u003c/i\u003e. Nitrosocosmicus” members are the dominant archaea associated with pepper (\u003ci\u003eCapsicum annuum\u003c/i\u003eL.) and ginseng (\u003ci\u003ePanax ginseng\u003c/i\u003eC.A. Mey.) plants’ rhizospheres.\n\u003ci\u003e\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1101/2024.01.08.574571\" target=\"_blank\"\u003eDOI: 10.1101/2024.01.08.574571\u003c/a\u003e\n","created_at":"2024-01-09T23:00:18.972Z","updated_at":"2025-11-06T14:36:35.659Z","authors":[{"id":13274,"given":"Ui-Ju","family":"Lee","created_at":"2024-01-09T23:00:18.975Z","updated_at":"2024-01-09T23:00:18.975Z","url":"https://api.seqco.de/v1/authors/13274.json"},{"id":5646,"given":"Joo-Han","family":"Gwak","created_at":"2019-04-15T19:29:38.005Z","updated_at":"2019-04-15T19:29:38.005Z","url":"https://api.seqco.de/v1/authors/5646.json"},{"id":13275,"given":"Seungyeon","family":"Choi","created_at":"2024-01-09T23:00:18.990Z","updated_at":"2024-01-09T23:00:18.990Z","url":"https://api.seqco.de/v1/authors/13275.json"},{"id":5645,"given":"Man-Young","family":"Jung","created_at":"2019-04-15T19:29:37.992Z","updated_at":"2019-04-15T19:29:37.992Z","url":"https://api.seqco.de/v1/authors/5645.json"},{"id":13276,"given":"Tae Kwon","family":"Lee","created_at":"2024-01-09T23:00:19.003Z","updated_at":"2024-01-09T23:00:19.003Z","url":"https://api.seqco.de/v1/authors/13276.json"},{"id":13277,"given":"Hojin","family":"Ryu","created_at":"2024-01-09T23:00:19.011Z","updated_at":"2024-01-09T23:00:19.011Z","url":"https://api.seqco.de/v1/authors/13277.json"},{"id":13278,"given":"Samuel Imisi","family":"Awala","created_at":"2024-01-09T23:00:19.020Z","updated_at":"2024-01-09T23:00:19.020Z","url":"https://api.seqco.de/v1/authors/13278.json"},{"id":13279,"given":"Wolfgang","family":"Wanek","created_at":"2024-01-09T23:00:19.028Z","updated_at":"2024-01-09T23:00:19.028Z","url":"https://api.seqco.de/v1/authors/13279.json"},{"id":324,"given":"Michael","family":"Wagner","created_at":"2019-04-15T18:46:01.846Z","updated_at":"2019-04-15T18:46:01.846Z","url":"https://api.seqco.de/v1/authors/324.json"},{"id":195,"given":"Zhe-Xue","family":"Quan","created_at":"2019-04-15T18:45:54.150Z","updated_at":"2019-04-15T18:45:54.150Z","url":"https://api.seqco.de/v1/authors/195.json"},{"id":5647,"given":"Sung-Keun","family":"Rhee","created_at":"2019-04-15T19:29:38.026Z","updated_at":"2019-04-15T19:29:38.026Z","url":"https://api.seqco.de/v1/authors/5647.json"}],"names":[],"subjects":[]}