{"response":{"status":"ok","message_type":"publication"},"id":3469,"citation":"König et al., 2016, Nature Microbiology","doi":"10.1038/nmicrobiol.2016.193","url":"https://api.seqco.de/v1/publications/3469.json","link_ext":"https://doi.org/10.1038/nmicrobiol.2016.193","title":"Nitrogen fixation in a chemoautotrophic lucinid symbiosis","journal":"Nature Microbiology","journal_loc":"2 (1)","journal_date":"2016-10-24","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003eThe shallow water bivalve \u003cjats:italic\u003eCodakia orbicularis\u003c/jats:italic\u003e lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO\u003cjats:sub\u003e2\u003c/jats:sub\u003e and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont–host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the \u003cjats:italic\u003eC. orbicularis\u003c/jats:italic\u003e symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The \u003cjats:italic\u003eC. orbicularis\u003c/jats:italic\u003e symbiont, here tentatively named ‘\u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Thiodiazotropha endolucinida’, may thus not only sustain the bivalve's carbon demands. \u003cjats:italic\u003eC. orbicularis\u003c/jats:italic\u003e may also benefit from a steady supply of fixed nitrogen from its symbiont—a scenario that is unprecedented in comparable chemoautotrophic symbioses.\u003c/jats:p\u003e","long_citation_html":"König et al. (2016). Nitrogen fixation in a chemoautotrophic lucinid symbiosis. \n\u003ci\u003eNature Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1038/nmicrobiol.2016.193\" target=\"_blank\"\u003eDOI:10.1038/nmicrobiol.2016.193\u003c/a\u003e\n","created_at":"2024-03-13T10:43:18.462Z","updated_at":"2025-11-06T14:38:15.375Z","authors":[{"id":13748,"given":"Sten","family":"König","created_at":"2024-03-13T10:43:18.512Z","updated_at":"2024-03-13T10:43:18.512Z","url":"https://api.seqco.de/v1/authors/13748.json"},{"id":8932,"given":"Olivier","family":"Gros","created_at":"2021-08-31T22:00:10.787Z","updated_at":"2021-08-31T22:00:10.787Z","url":"https://api.seqco.de/v1/authors/8932.json"},{"id":13749,"given":"Stefan E.","family":"Heiden","created_at":"2024-03-13T10:43:18.533Z","updated_at":"2024-03-13T10:43:18.533Z","url":"https://api.seqco.de/v1/authors/13749.json"},{"id":12725,"given":"Tjorven","family":"Hinzke","created_at":"2023-09-14T13:34:57.069Z","updated_at":"2023-09-14T13:34:57.069Z","url":"https://api.seqco.de/v1/authors/12725.json"},{"id":13750,"given":"Andrea","family":"Thürmer","created_at":"2024-03-13T10:43:18.549Z","updated_at":"2024-03-13T10:43:18.549Z","url":"https://api.seqco.de/v1/authors/13750.json"},{"id":2674,"given":"Anja","family":"Poehlein","created_at":"2019-04-15T18:47:28.879Z","updated_at":"2019-04-15T18:47:28.879Z","url":"https://api.seqco.de/v1/authors/2674.json"},{"id":13751,"given":"Susann","family":"Meyer","created_at":"2024-03-13T10:43:18.565Z","updated_at":"2024-03-13T10:43:18.565Z","url":"https://api.seqco.de/v1/authors/13751.json"},{"id":13752,"given":"Magalie","family":"Vatin","created_at":"2024-03-13T10:43:18.574Z","updated_at":"2024-03-13T10:43:18.574Z","url":"https://api.seqco.de/v1/authors/13752.json"},{"id":13753,"given":"Didier","family":"Mbéguié-A-Mbéguié","created_at":"2024-03-13T10:43:18.585Z","updated_at":"2024-03-13T10:43:18.585Z","url":"https://api.seqco.de/v1/authors/13753.json"},{"id":13754,"given":"Jennifer","family":"Tocny","created_at":"2024-03-13T10:43:18.594Z","updated_at":"2024-03-13T10:43:18.594Z","url":"https://api.seqco.de/v1/authors/13754.json"},{"id":13755,"given":"Ruby","family":"Ponnudurai","created_at":"2024-03-13T10:43:18.603Z","updated_at":"2024-03-13T10:43:18.603Z","url":"https://api.seqco.de/v1/authors/13755.json"},{"id":2675,"given":"Rolf","family":"Daniel","created_at":"2019-04-15T18:47:28.893Z","updated_at":"2019-04-15T18:47:28.893Z","url":"https://api.seqco.de/v1/authors/2675.json"},{"id":13756,"given":"Dörte","family":"Becher","created_at":"2024-03-13T10:43:18.617Z","updated_at":"2024-03-13T10:43:18.617Z","url":"https://api.seqco.de/v1/authors/13756.json"},{"id":10252,"given":"Thomas","family":"Schweder","created_at":"2022-04-09T22:00:09.053Z","updated_at":"2022-04-09T22:00:09.053Z","url":"https://api.seqco.de/v1/authors/10252.json"},{"id":10250,"given":"Stephanie","family":"Markert","created_at":"2022-04-09T22:00:09.023Z","updated_at":"2022-04-09T22:00:09.023Z","url":"https://api.seqco.de/v1/authors/10250.json"}],"names":[{"id":41622,"name":"Thiodiazotropha endolucinida","url":"https://api.seqco.de/v1/names/41622.json","uri":"https://seqco.de/i:41622"},{"id":44042,"name":"Candidatus Thiodiazotropha fergusoni","url":"https://api.seqco.de/v1/names/44042.json","uri":"https://seqco.de/i:44042"}],"subjects":[{"id":23,"name":"Applied Microbiology and Biotechnology","url":"https://api.seqco.de/v1/subjects/23.json"},{"id":30,"name":"Cell Biology","url":"https://api.seqco.de/v1/subjects/30.json"},{"id":15,"name":"Genetics","url":"https://api.seqco.de/v1/subjects/15.json"},{"id":10,"name":"Immunology","url":"https://api.seqco.de/v1/subjects/10.json"},{"id":9,"name":"Microbiology","url":"https://api.seqco.de/v1/subjects/9.json"},{"id":8,"name":"Microbiology (medical)","url":"https://api.seqco.de/v1/subjects/8.json"}]}