{"response":{"status":"ok","message_type":"publication"},"id":3514,"citation":"Aksenov et al., 2024, ","doi":"10.21203/rs.3.rs-4224869/v1","url":"https://api.seqco.de/v1/publications/3514.json","link_ext":"https://doi.org/10.21203/rs.3.rs-4224869/v1","title":"Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus ","journal":"","journal_loc":"","journal_date":"2024-04-17","pub_type":"posted-content","abstract":"\u003ctitle\u003eAbstract\u003c/title\u003e\n        \u003cp\u003eHuanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, \u003citalic\u003eCandidatus\u003c/italic\u003e Liberibacter asiaticus \u003citalic\u003e(C\u003c/italic\u003eLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change \u003citalic\u003ein vivo\u003c/italic\u003e in HLB-impacted trees. We demonstrate how spatial information from molecular maps of branches and single leaves yields insight into the biology not accessible otherwise. In particular, we found evidence that flavonoid biosynthesis is disrupted in HLB-impacted trees, and an increase in the polyamine, feruloylputrescine, is highly correlated with an increase in disease severity. Based on mechanistic details revealed by these molecular maps, followed by metabolic modeling, we formulated and tested the hypothesis that \u003citalic\u003eC\u003c/italic\u003eLas infection either directly or indirectly converts the precursor compound, ferulic acid, to feruloylputrescine to suppress the antimicrobial effects of ferulic acid and biosynthetically downstream flavonoids. Using \u003citalic\u003ein vitro\u003c/italic\u003e bioassays, we demonstrated that ferulic acid and bioflavonoids are indeed highly bactericidal to \u003citalic\u003eC\u003c/italic\u003eLas, with the activity on par with a reference antibiotic, oxytetracycline, recently approved for HLB management. We propose these compounds should be evaluated as therapeutics alternatives to the antibiotics for HLB treatment. Overall, the utilized 3D metabolic mapping approach provides a promising methodological framework to identify pathogen-specific inhibitory compounds \u003citalic\u003ein planta\u003c/italic\u003e for potential prophylactic or therapeutic applications.\u003c/p\u003e","long_citation_html":"Aksenov et al. (2024). Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus .\n\u003ci\u003e\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.21203/rs.3.rs-4224869/v1\" target=\"_blank\"\u003eDOI: 10.21203/rs.3.rs-4224869/v1\u003c/a\u003e\n","created_at":"2024-04-18T22:00:55.586Z","updated_at":"2025-11-06T14:38:46.806Z","authors":[{"id":13901,"given":"Alexander A.","family":"Aksenov","created_at":"2024-04-18T22:00:55.610Z","updated_at":"2024-04-18T22:00:55.610Z","url":"https://api.seqco.de/v1/authors/13901.json"},{"id":7244,"given":"Alex","family":"Blacutt","created_at":"2020-02-23T07:30:40.397Z","updated_at":"2020-02-23T07:30:40.397Z","url":"https://api.seqco.de/v1/authors/7244.json"},{"id":7245,"given":"Nichole","family":"Ginnan","created_at":"2020-02-23T07:30:40.622Z","updated_at":"2020-02-23T07:30:40.622Z","url":"https://api.seqco.de/v1/authors/7245.json"},{"id":5799,"given":"Philippe","family":"Rolshausen","created_at":"2019-04-15T19:29:42.292Z","updated_at":"2019-04-15T19:29:42.292Z","url":"https://api.seqco.de/v1/authors/5799.json"},{"id":13871,"given":"Alexey V.","family":"Melnik","created_at":"2024-04-16T22:00:33.948Z","updated_at":"2024-04-16T22:00:33.948Z","url":"https://api.seqco.de/v1/authors/13871.json"},{"id":13872,"given":"Ali","family":"Lotfi","created_at":"2024-04-16T22:00:33.957Z","updated_at":"2024-04-16T22:00:33.957Z","url":"https://api.seqco.de/v1/authors/13872.json"},{"id":13873,"given":"Emily C.","family":"Gentry","created_at":"2024-04-16T22:00:33.969Z","updated_at":"2024-04-16T22:00:33.969Z","url":"https://api.seqco.de/v1/authors/13873.json"},{"id":7939,"given":"Manikandan","family":"Ramasamy","created_at":"2020-12-14T19:08:26.652Z","updated_at":"2020-12-14T19:08:26.652Z","url":"https://api.seqco.de/v1/authors/7939.json"},{"id":13874,"given":"Cristal","family":"Zuniga","created_at":"2024-04-16T22:00:33.985Z","updated_at":"2024-04-16T22:00:33.985Z","url":"https://api.seqco.de/v1/authors/13874.json"},{"id":7649,"given":"Karsten","family":"Zengler","created_at":"2020-09-04T00:54:51.893Z","updated_at":"2020-09-04T00:54:51.893Z","url":"https://api.seqco.de/v1/authors/7649.json"},{"id":10796,"given":"Kranthi","family":"Mandadi","created_at":"2022-10-06T22:00:26.638Z","updated_at":"2022-10-06T22:00:26.638Z","url":"https://api.seqco.de/v1/authors/10796.json"},{"id":13902,"given":"Pieter","family":"Dorrestein","created_at":"2024-04-18T22:00:55.854Z","updated_at":"2024-04-18T22:00:55.854Z","url":"https://api.seqco.de/v1/authors/13902.json"},{"id":5798,"given":"Caroline","family":"Roper","created_at":"2019-04-15T19:29:42.285Z","updated_at":"2019-04-15T19:29:42.285Z","url":"https://api.seqco.de/v1/authors/5798.json"}],"names":[{"id":1,"name":"Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[]}