{"response":{"status":"ok","message_type":"publication"},"id":3577,"citation":"Cai, Dong, 2010, International Journal of Systematic and Evolutionary Microbiology","doi":"10.1099/ijs.0.014712-0","url":"https://api.seqco.de/v1/publications/3577.json","link_ext":"https://doi.org/10.1099/ijs.0.014712-0","title":"Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov.","journal":"International Journal of Systematic and Evolutionary Microbiology","journal_loc":"60 (4)","journal_date":"2010-04-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003eAn obligate anaerobic, Gram-staining-negative, mesophilic, cellulolytic bacterium, strain H1\u003cjats:sup\u003eT\u003c/jats:sup\u003e, was isolated from the rumen content of yak. Cells were straight to slightly curved rods, 0.8–1.0×3.0–4.0 μm in size, non-motile and encapsulated with mucous materials. Elliptical and terminal spores that swelled the cells were produced occasionally. The strain grew at 25–45 °C (optimum, 38 °C) and pH 6.0–7.8 (optimum, pH 6.7). Cellulose, cellobiose, xylan, xylose and maltose were used as carbon and energy sources, but not glucose. Products from cellulose and cellobiose fermentation were formic acid, acetic acid, carbon dioxide and trace amounts of ethanol, lactic acid and succinic acid. The genomic DNA G+C content was 33.7±1.2 mol%. The predominant fatty acids were C\u003cjats:sub\u003e16 : 0\u003c/jats:sub\u003e(27.1 %), C\u003cjats:sub\u003e14 : 0\u003c/jats:sub\u003e(9.2 %) and iso-C\u003cjats:sub\u003e16 : 0\u003c/jats:sub\u003e(6.4%). Based on the 16S rRNA gene sequence analysis, strain H1\u003cjats:sup\u003eT\u003c/jats:sup\u003ewas affiliated to the clostridial rRNA cluster XIVb and showed the highest 16S rRNA gene sequence similarity to\u003cjats:italic\u003eClostridium lentocellum\u003c/jats:italic\u003eDSM 5427\u003cjats:sup\u003eT\u003c/jats:sup\u003e(96.0 %). These two strains formed a distinct lineage of the family ‘\u003cjats:italic\u003eLachnospiraceae\u003c/jats:italic\u003e’. Based on data from this polyphasic taxonomic study, a new genus,\u003cjats:italic\u003eCellulosilyticum\u003c/jats:italic\u003egen. nov., is proposed.\u003cjats:italic\u003eCellulosilyticum ruminicola\u003c/jats:italic\u003esp. nov. is proposed for strain H1\u003cjats:sup\u003eT\u003c/jats:sup\u003e. The type strain of\u003cjats:italic\u003eCellulosilyticum ruminicola\u003c/jats:italic\u003esp. nov. is strain H1\u003cjats:sup\u003eT\u003c/jats:sup\u003e(=CGMCC 1.5065\u003cjats:sup\u003eT\u003c/jats:sup\u003e=JCM 14822\u003cjats:sup\u003eT\u003c/jats:sup\u003e).\u003cjats:italic\u003eClostridium lentocellum\u003c/jats:italic\u003ewas reclassified in the new genus as\u003cjats:italic\u003eCellulosilyticum lentocellum\u003c/jats:italic\u003ecomb. nov. (type strain RHM5\u003cjats:sup\u003eT\u003c/jats:sup\u003e=ATCC 49066\u003cjats:sup\u003eT\u003c/jats:sup\u003e=DSM 5427\u003cjats:sup\u003eT\u003c/jats:sup\u003e=NCIMB 11756\u003cjats:sup\u003eT\u003c/jats:sup\u003e).\u003c/jats:p\u003e","long_citation_html":"Cai, Dong (2010). Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov.\n\u003ci\u003eInternational Journal of Systematic and Evolutionary Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1099/ijs.0.014712-0\" target=\"_blank\"\u003eDOI: 10.1099/ijs.0.014712-0\u003c/a\u003e\n","created_at":"2024-05-21T16:06:30.083Z","updated_at":"2025-11-06T14:39:33.559Z","authors":[{"id":14100,"given":"Shichun","family":"Cai","created_at":"2024-05-21T16:06:30.093Z","updated_at":"2024-05-21T16:06:30.093Z","url":"https://api.seqco.de/v1/authors/14100.json"},{"id":9811,"given":"Xiuzhu","family":"Dong","created_at":"2021-12-24T23:00:11.747Z","updated_at":"2021-12-24T23:00:11.747Z","url":"https://api.seqco.de/v1/authors/9811.json"}],"names":[],"subjects":[]}