{"response":{"status":"ok","message_type":"publication"},"id":38,"citation":"Rubio-Cabetas, Sancho, 1970, Spanish Journal of Agricultural Research","doi":"10.5424/sjar/2009072-1503","url":"https://api.seqco.de/v1/publications/38.json","link_ext":"https://doi.org/10.5424/sjar/2009072-1503","title":"Detection and identification of 'Candidatus Phytoplasma prunorum'in Prunus germplasm","journal":"Spanish Journal of Agricultural Research","journal_loc":"7 (2)","journal_date":"1970-01-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003eThe molecular characterization of the causal agent of diseases associated with several symptoms such as decline, yellowing,leaf roll and off-season growth in stone fruits made it possible to determine a common etiology, and the name ´European stone fruit yellows´ phytoplasma was proposed. Recently, the new taxonomical species description within the genus is 'Candidatus Phytoplasma prunorum'.A 2-yr survey was carried out in two different Prunus collections of the Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA) including European and Japanese plum genotypes of various species and several interspecific hybrids used as rootstocks. Both off-season growth in winter and decline were observed. In order to identify the phytoplasma suspected as a causal agent, two different PCR methods were applied to all inspected trees. The first method was a nested PCR with 16Sr X group-specific primers followed by RFLP analysis. Thesecond method was a direct PCR with specific primers for 'Ca P. prunorum' (Eca1/Eca2). In the most symptomatic treesthe presence of this phytoplasma was confirmed by at least one of the methods; negative results were obtained in asymptomatic trees. The nested PCR-RFLPs analysis was confirmed as a reliable method for routinary diagnosis rather than direct PCR.\u003c/jats:p\u003e","long_citation_html":"Rubio-Cabetas, Sancho (1970). Detection and identification of \u0026#39;Candidatus Phytoplasma prunorum\u0026#39;in Prunus germplasm. \n\u003ci\u003eSpanish Journal of Agricultural Research\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.5424/sjar/2009072-1503\" target=\"_blank\"\u003eDOI: 10.5424/sjar/2009072-1503\u003c/a\u003e\n","created_at":"2019-04-15T18:45:43.461Z","updated_at":"2025-11-06T11:24:51.621Z","authors":[{"id":130,"given":"M. J.","family":"Rubio-Cabetas","created_at":"2019-04-15T18:45:43.471Z","updated_at":"2019-04-15T18:45:43.471Z","url":"https://api.seqco.de/v1/authors/130.json"},{"id":131,"given":"S.","family":"Sancho","created_at":"2019-04-15T18:45:43.485Z","updated_at":"2019-04-15T18:45:43.485Z","url":"https://api.seqco.de/v1/authors/131.json"}],"names":[{"id":67,"name":"Candidatus Phytoplasma prunorum","url":"https://api.seqco.de/v1/names/67.json","uri":"https://seqco.de/i:67"}],"subjects":[{"id":5,"name":"Agronomy and Crop Science","url":"https://api.seqco.de/v1/subjects/5.json"}]}