{"response":{"status":"ok","message_type":"publication"},"id":3847,"citation":"Roldán et al., 2025, Pest Management Science","doi":"10.1002/ps.8552","url":"https://api.seqco.de/v1/publications/3847.json","link_ext":"https://doi.org/10.1002/ps.8552","title":"Evaluation of tree‐injected oxytetracycline and antisense oligonucleotides targeting \u003cscp\u003e\u003ci\u003eCandidatus\u003c/i\u003e\u003c/scp\u003e Liberibacter asiaticus in citrus","journal":"Pest Management Science","journal_loc":"81 (3)","journal_date":"2025-03-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:sec\u003e\u003cjats:title\u003eBACKGROUND\u003c/jats:title\u003e\u003cjats:p\u003eHuanglongbing (HLB) is a systemic disease of citrus caused by the bacterial pathogen \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Liberibacter asiaticus (\u003cjats:italic\u003eC\u003c/jats:italic\u003eLas) that limits citrus production worldwide. \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas is an obligate bacterial pathogen that multiplies in citrus trees and in the insect vector, the Asian citrus psyllid (ACP), \u003cjats:italic\u003eDiaphorina citri\u003c/jats:italic\u003e Kuwayama. There is no cure for HLB currently and broad‐spectrum antibiotics represent one possible therapeutic against disease symptoms. Single‐stranded nucleic acid analogs, 2′‐deoxy‐2’‐Fluoro‐β‐D‐arabinonucleic antisense oligonucleotides (FANA ASOs), can modulate gene expression by enzymatic degradation or steric blocking of an RNA target. FANA ASOs recognize and bind to specific RNA forms, including mRNA, miRNA and long noncoding RNA, through complementary base‐pairing.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eRESULTS\u003c/jats:title\u003e\u003cjats:p\u003eInjection of oxytetracycline (OTC) into mature citrus trees with HLB ameliorated symptoms of disease, increased fruit yield and quality of juice as compared with that produced by noninjected controls. Injection of trees with FANA ASOs reduced \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas abundance but did not improve fruit yield and quality above control levels at the injection dosage tested. Reduced pathogen relative abundance following OTC injection was coincident with lower \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas acquisition and inoculation by laboratory deployed and wild‐type \u003cjats:italic\u003eD. citri\u003c/jats:italic\u003e collected from the field, respectively. However, injections of FANA ASOs did not consistently reduce \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas transmission as compared with noninjected controls.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eCONCLUSIONS\u003c/jats:title\u003e\u003cjats:p\u003eTrunk injection of OTC may be useful in management of HLB by reducing \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas infection in trees and disrupting transmission. However, more research is needed to verify the potential usefulness of FANA ASOs. Optimizing FANA ASO dosage in trees and exploring the potential of multiplex FANA ASOs that simultaneously target multiple mRNAs could possibly enhance their efficacy against \u003cjats:italic\u003eC\u003c/jats:italic\u003eLas. © 2024 Society of Chemical Industry.\u003c/jats:p\u003e\u003c/jats:sec\u003e","long_citation_html":"Roldán et al. (2025). Evaluation of tree‐injected oxytetracycline and antisense oligonucleotides targeting \u0026lt;scp\u0026gt;\u003ci\u003eCandidatus\u003c/i\u003e\u0026lt;/scp\u0026gt; Liberibacter asiaticus in citrus. \n\u003ci\u003ePest Management Science\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1002/ps.8552\" target=\"_blank\"\u003eDOI:10.1002/ps.8552\u003c/a\u003e\n","created_at":"2024-11-26T23:00:19.621Z","updated_at":"2025-11-06T11:19:10.420Z","authors":[{"id":15107,"given":"Erik L.","family":"Roldán","created_at":"2024-11-26T23:00:19.625Z","updated_at":"2024-11-26T23:00:19.625Z","url":"https://api.seqco.de/v1/authors/15107.json"},{"id":1544,"given":"Lukasz L.","family":"Stelinski","created_at":"2019-04-15T18:46:42.438Z","updated_at":"2019-04-15T18:46:42.438Z","url":"https://api.seqco.de/v1/authors/1544.json"},{"id":15108,"given":"Kirsten S.","family":"Pelz‐Stelinski","created_at":"2024-11-26T23:00:19.675Z","updated_at":"2024-11-26T23:00:19.675Z","url":"https://api.seqco.de/v1/authors/15108.json"}],"names":[{"id":1,"name":"Candidatus Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[]}