{"response":{"status":"ok","message_type":"publication"},"id":3848,"citation":"Checchia et al., 2025, Pest Management Science","doi":"10.1002/ps.8564","url":"https://api.seqco.de/v1/publications/3848.json","link_ext":"https://doi.org/10.1002/ps.8564","title":"Testing low‐risk bioactive compounds on \u003ci\u003eHalyomorpha halys\u003c/i\u003e: an improved pipeline of analyses to investigate their effects on the bacterial endosymbiont \u003ci\u003eCandidatus Pantoea carbekii\u003c/i\u003e","journal":"Pest Management Science","journal_loc":"81 (3)","journal_date":"2025-03-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:sec\u003e\u003cjats:title\u003eBACKGROUND\u003c/jats:title\u003e\u003cjats:p\u003eThe brown marmorated stink bug \u003cjats:italic\u003eHalyomorpha halys\u003c/jats:italic\u003e has become an invasive insect pest of many crops. A promising control strategy to manage the proliferation of \u003cjats:italic\u003eH. halys\u003c/jats:italic\u003e is based on the suppression of its obligate and vertically transmitted uncultivated symbiotic bacterium \u003cjats:italic\u003eCandidatus Pantoea carbekii\u003c/jats:italic\u003e through surface‐sterilization of \u003cjats:italic\u003eH\u003c/jats:italic\u003e. \u003cjats:italic\u003ehalys\u003c/jats:italic\u003e eggs. Indeed, the application of antimicrobial formulations on the eggs of \u003cjats:italic\u003eH\u003c/jats:italic\u003e. \u003cjats:italic\u003ehalys\u003c/jats:italic\u003e could cause mortality of endosymbiont and consequently of newly emerged nymphs.\u003c/jats:p\u003e\u003cjats:p\u003eIn this study, a microbial live/dead assay was applied directly on \u003cjats:italic\u003eH\u003c/jats:italic\u003e. \u003cjats:italic\u003ehalys\u003c/jats:italic\u003e eggs to evaluate \u003cjats:italic\u003eCa\u003c/jats:italic\u003e. \u003cjats:italic\u003eP. carbekii\u003c/jats:italic\u003e loss of viability after treatments with seven commercial formulations including fungicides (copper hydroxide, sulphur, sweet orange essential oil) and plant biostimulants (flavonoids and chestnut tannin extract) compared with two disinfectants for civil and industrial use (sodium hypochlorite/hydrated sodium/tetraborate decahydrate and peracetic acid/hydrogen peroxide). Impact of mode of application was also evaluated, as surface treatment of egg masses was performed through spraying and dipping in laboratory conditions. Antimicrobial activity data were finally complemented with observations of egg hatching and vitality of the nymphs.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eRESULTS\u003c/jats:title\u003e\u003cjats:p\u003eThe optimization of live/dead staining is useful for evaluating \u003cjats:italic\u003eCa\u003c/jats:italic\u003e. \u003cjats:italic\u003eP. carbekii\u003c/jats:italic\u003e mortality directly on eggs, providing a rapid and reliable culture‐independent approach. Sodium hypochlorite, copper, sulphur, tannins and sweet orange essential oil showed an antimicrobial effect against \u003cjats:italic\u003eCa\u003c/jats:italic\u003e. \u003cjats:italic\u003eP. carbekii\u003c/jats:italic\u003e and a \u003cjats:italic\u003eH. halys\u003c/jats:italic\u003e egg hatching reduction and nymph's vitality.\u003c/jats:p\u003e\u003c/jats:sec\u003e\u003cjats:sec\u003e\u003cjats:title\u003eCONCLUSIONS\u003c/jats:title\u003e\u003cjats:p\u003eThe antimicrobial and insecticidal effects of these commercial products should be further studied to assess their in‐field efficiency as well as the impact of these substances on non‐target organisms. The approach followed in this study could be considered a robust pipeline of analyses to evaluate the effectiveness of antimicrobial eco‐friendly compounds in symbiotic control of \u003cjats:italic\u003eH\u003c/jats:italic\u003e. \u003cjats:italic\u003ehalys\u003c/jats:italic\u003e. © 2024 Society of Chemical Industry.\u003c/jats:p\u003e\u003c/jats:sec\u003e","long_citation_html":"Checchia et al. (2025). Testing low‐risk bioactive compounds on \u003ci\u003eHalyomorpha halys\u003c/i\u003e: an improved pipeline of analyses to investigate their effects on the bacterial endosymbiont \u003ci\u003eCandidatus Pantoea carbekii\u003c/i\u003e. \n\u003ci\u003ePest Management Science\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1002/ps.8564\" target=\"_blank\"\u003eDOI: 10.1002/ps.8564\u003c/a\u003e\n","created_at":"2024-11-26T23:00:19.686Z","updated_at":"2025-11-06T11:19:12.858Z","authors":[{"id":15109,"given":"Ilaria","family":"Checchia","created_at":"2024-11-26T23:00:19.690Z","updated_at":"2024-11-26T23:00:19.690Z","url":"https://api.seqco.de/v1/authors/15109.json"},{"id":15110,"given":"Marco","family":"Andreolli","created_at":"2024-11-26T23:00:19.697Z","updated_at":"2024-11-26T23:00:19.697Z","url":"https://api.seqco.de/v1/authors/15110.json"},{"id":15111,"given":"Francesco","family":"Lanza","created_at":"2024-11-26T23:00:19.704Z","updated_at":"2024-11-26T23:00:19.704Z","url":"https://api.seqco.de/v1/authors/15111.json"},{"id":14896,"given":"Giacomo","family":"Santoiemma","created_at":"2024-09-28T22:00:28.262Z","updated_at":"2024-09-28T22:00:28.262Z","url":"https://api.seqco.de/v1/authors/14896.json"},{"id":6778,"given":"Nicola","family":"Mori","created_at":"2019-09-01T06:31:04.993Z","updated_at":"2019-09-01T06:31:04.993Z","url":"https://api.seqco.de/v1/authors/6778.json"},{"id":15112,"given":"Massimiliano","family":"Pasini","created_at":"2024-11-26T23:00:19.722Z","updated_at":"2024-11-26T23:00:19.722Z","url":"https://api.seqco.de/v1/authors/15112.json"},{"id":15113,"given":"Silvia","family":"Lampis","created_at":"2024-11-26T23:00:19.730Z","updated_at":"2024-11-26T23:00:19.730Z","url":"https://api.seqco.de/v1/authors/15113.json"},{"id":15114,"given":"Giovanna E","family":"Felis","created_at":"2024-11-26T23:00:19.738Z","updated_at":"2024-11-26T23:00:19.738Z","url":"https://api.seqco.de/v1/authors/15114.json"}],"names":[{"id":38,"name":"Candidatus Pantoea carbekii","url":"https://api.seqco.de/v1/names/38.json","uri":"https://seqco.de/i:38"}],"subjects":[]}