{"response":{"status":"ok","message_type":"publication"},"id":4225,"citation":"Kochetkova et al., 2020, International Journal of Systematic and Evolutionary Microbiology","doi":"10.1099/ijsem.0.003902","url":"https://api.seqco.de/v1/publications/4225.json","link_ext":"https://doi.org/10.1099/ijsem.0.003902","title":"Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14","journal":"International Journal of Systematic and Evolutionary Microbiology","journal_loc":"70 (2)","journal_date":"2020-02-01","pub_type":"journal-article","abstract":"\u003cjats:p\u003eA novel aerobic moderately thermophilic bacterium, strain 3753O\u003cjats:sup\u003eT\u003c/jats:sup\u003e, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753O\u003cjats:sup\u003eT\u003c/jats:sup\u003e grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO\u003cjats:sub\u003e3\u003c/jats:sub\u003e) as the electron donor. The major cellular fatty acid was branched C\u003cjats:sub\u003e19 : 0\u003c/jats:sub\u003e. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753O\u003cjats:sup\u003eT\u003c/jats:sup\u003e was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753O\u003cjats:sup\u003eT\u003c/jats:sup\u003e within the previously uncultivated lineage OLB14 in the phylum \u003cjats:italic\u003e\n                     \u003cjats:named-content content-type=\"class\"\u003e\n                        \u003cjats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"uri\" xlink:href=\"http://doi.org/10.1601/nm.550\" xlink:type=\"simple\"\u003eChloroflexi\u003c/jats:ext-link\u003e\n                     \u003c/jats:named-content\u003e\n                  \u003c/jats:italic\u003e. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species \u003cjats:italic\u003eTepidiforma bonchosmolovskayae\u003c/jats:italic\u003e gen. nov.\u003cjats:italic\u003e,\u003c/jats:italic\u003e sp. nov., within the \u003cjats:italic\u003eTepidiformaceae\u003c/jats:italic\u003e fam. nov., the \u003cjats:italic\u003eTepidiformales\u003c/jats:italic\u003e ord. nov. and the \u003cjats:italic\u003eTepidiformia\u003c/jats:italic\u003e classis nov. are proposed. The type strain of \u003cjats:italic\u003eTepidiforma bonchosmolovskayae\u003c/jats:italic\u003e is 3753O\u003cjats:sup\u003eT\u003c/jats:sup\u003e (=VKM B-3389\u003cjats:sup\u003eT\u003c/jats:sup\u003e=KTCT 72284\u003cjats:sup\u003eT\u003c/jats:sup\u003e).\u003c/jats:p\u003e","long_citation_html":"Kochetkova et al. (2020). Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14. \n\u003ci\u003eInternational Journal of Systematic and Evolutionary Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1099/ijsem.0.003902\" target=\"_blank\"\u003eDOI:10.1099/ijsem.0.003902\u003c/a\u003e\n","created_at":"2025-08-29T13:13:44.643Z","updated_at":"2025-11-06T14:46:27.704Z","authors":[{"id":16692,"given":"Tatiana V.","family":"Kochetkova","created_at":"2025-08-29T13:13:44.727Z","updated_at":"2025-08-29T13:13:44.727Z","url":"https://api.seqco.de/v1/authors/16692.json"},{"id":16471,"given":"Kseniya S.","family":"Zayulina","created_at":"2025-08-28T12:06:34.901Z","updated_at":"2025-08-28T12:06:34.901Z","url":"https://api.seqco.de/v1/authors/16471.json"},{"id":16693,"given":"Vyacheslav S.","family":"Zhigarkov","created_at":"2025-08-29T13:13:45.170Z","updated_at":"2025-08-29T13:13:45.170Z","url":"https://api.seqco.de/v1/authors/16693.json"},{"id":16694,"given":"Nikita V.","family":"Minaev","created_at":"2025-08-29T13:13:45.183Z","updated_at":"2025-08-29T13:13:45.183Z","url":"https://api.seqco.de/v1/authors/16694.json"},{"id":16695,"given":"Boris N.","family":"Chichkov","created_at":"2025-08-29T13:13:45.195Z","updated_at":"2025-08-29T13:13:45.195Z","url":"https://api.seqco.de/v1/authors/16695.json"},{"id":13999,"given":"Andrei A.","family":"Novikov","created_at":"2024-05-11T18:43:57.150Z","updated_at":"2024-05-11T18:43:57.150Z","url":"https://api.seqco.de/v1/authors/13999.json"},{"id":12524,"given":"Stepan V.","family":"Toshchakov","created_at":"2023-08-19T18:53:11.854Z","updated_at":"2023-08-19T18:53:11.854Z","url":"https://api.seqco.de/v1/authors/12524.json"},{"id":13998,"given":"Alexander G.","family":"Elcheninov","created_at":"2024-05-11T18:43:57.139Z","updated_at":"2024-05-11T18:43:57.139Z","url":"https://api.seqco.de/v1/authors/13998.json"},{"id":11803,"given":"Ilya V.","family":"Kublanov","created_at":"2023-06-16T17:29:34.786Z","updated_at":"2023-06-16T17:29:34.786Z","url":"https://api.seqco.de/v1/authors/11803.json"}],"names":[{"id":21177,"name":"Tepidiformia","url":"https://api.seqco.de/v1/names/21177.json","uri":"https://seqco.de/i:21177"},{"id":21178,"name":"Tepidiformales","url":"https://api.seqco.de/v1/names/21178.json","uri":"https://seqco.de/i:21178"},{"id":21179,"name":"Tepidiformaceae","url":"https://api.seqco.de/v1/names/21179.json","uri":"https://seqco.de/i:21179"}],"subjects":[]}