{"response":{"status":"ok","message_type":"publication"},"id":4790,"citation":"Kondrotaite et al., 2022, Frontiers in Microbiology","doi":"10.3389/fmicb.2022.917553","url":"https://api.seqco.de/v1/publications/4790.json","link_ext":"https://doi.org/10.3389/fmicb.2022.917553","title":"Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems","journal":"Frontiers in Microbiology","journal_loc":"13","journal_date":"2022-07-08","pub_type":"journal-article","abstract":"\u003cjats:p\u003eThe \u003cjats:italic\u003eSaprospiraceae\u003c/jats:italic\u003e family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of \u003cjats:italic\u003eSaprospiraceae\u003c/jats:italic\u003e. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence \u003cjats:italic\u003ein situ\u003c/jats:italic\u003e hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Brachybacter, \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Parvibacillus calidus (both from the former genus OLB8), and \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Opimibacter, and the presence of polyhydroxyalkanoates in \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Defluviibacterium haderslevense and \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e Vicinibacter. These results provide the first overview of the most abundant novel \u003cjats:italic\u003eSaprospiraceae\u003c/jats:italic\u003e genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.\u003c/jats:p\u003e","long_citation_html":"Kondrotaite et al. (2022). Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems.\n\u003ci\u003eFrontiers in Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.3389/fmicb.2022.917553\" target=\"_blank\"\u003eDOI: 10.3389/fmicb.2022.917553\u003c/a\u003e\n","created_at":"2026-09-01T07:01:56.058Z","updated_at":"2026-10-02T10:35:01.476Z","authors":[{"id":7524,"given":"Zivile","family":"Kondrotaite","created_at":"2020-06-14T06:31:03.471Z","updated_at":"2020-06-14T06:31:03.471Z","url":"https://api.seqco.de/v1/authors/7524.json"},{"id":5661,"given":"Laura C.","family":"Valk","created_at":"2019-04-15T19:29:38.214Z","updated_at":"2019-04-15T19:29:38.214Z","url":"https://api.seqco.de/v1/authors/5661.json"},{"id":7908,"given":"Francesca","family":"Petriglieri","created_at":"2020-12-14T19:04:52.541Z","updated_at":"2020-12-14T19:04:52.541Z","url":"https://api.seqco.de/v1/authors/7908.json"},{"id":7909,"given":"Caitlin","family":"Singleton","created_at":"2020-12-14T19:04:52.882Z","updated_at":"2020-12-14T19:04:52.882Z","url":"https://api.seqco.de/v1/authors/7909.json"},{"id":6198,"given":"Marta","family":"Nierychlo","created_at":"2019-04-15T19:30:14.366Z","updated_at":"2019-04-15T19:30:14.366Z","url":"https://api.seqco.de/v1/authors/6198.json"},{"id":9793,"given":"Morten K. D.","family":"Dueholm","created_at":"2021-12-21T23:00:10.222Z","updated_at":"2021-12-21T23:00:10.222Z","url":"https://api.seqco.de/v1/authors/9793.json"},{"id":3863,"given":"Per H.","family":"Nielsen","created_at":"2019-04-15T19:19:36.145Z","updated_at":"2019-04-15T19:19:36.145Z","url":"https://api.seqco.de/v1/authors/3863.json"}],"names":[{"id":44863,"name":"Parvibacillus calidus","url":"https://api.seqco.de/v1/names/44863.json","uri":"https://seqco.de/i:44863"},{"id":44888,"name":"Vicinibacter","url":"https://api.seqco.de/v1/names/44888.json","uri":"https://seqco.de/i:44888"},{"id":45025,"name":"Opimibacter","url":"https://api.seqco.de/v1/names/45025.json","uri":"https://seqco.de/i:45025"},{"id":45026,"name":"Opimibacter skivensis","url":"https://api.seqco.de/v1/names/45026.json","uri":"https://seqco.de/i:45026"},{"id":45140,"name":"Opimibacter iunctus","url":"https://api.seqco.de/v1/names/45140.json","uri":"https://seqco.de/i:45140"},{"id":56584,"name":"Candidatus Brachybacter","url":"https://api.seqco.de/v1/names/56584.json","uri":"https://seqco.de/i:56584"},{"id":56585,"name":"Defluviibacterium haderslevense","url":"https://api.seqco.de/v1/names/56585.json","uri":"https://seqco.de/i:56585"}],"subjects":[]}