{"response":{"status":"ok","message_type":"publication"},"id":4927,"citation":"Yang et al., 2017, The ISME Journal","doi":"10.1038/ismej.2017.127","url":"https://api.seqco.de/v1/publications/4927.json","link_ext":"https://doi.org/10.1038/ismej.2017.127","title":"Grape pomace compost harbors organohalide-respiring \u003ci\u003eDehalogenimonas\u003c/i\u003e species with novel reductive dehalogenase genes","journal":"The ISME Journal","journal_loc":"11 (12)","journal_date":"2017-12-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\n               \u003cjats:p\u003eOrganohalide-respiring bacteria have key roles in the natural chlorine cycle; however, most of the current knowledge is based on cultures from contaminated environments. We demonstrate that grape pomace compost without prior exposure to chlorinated solvents harbors a Dehalogenimonas (Dhgm) species capable of using chlorinated ethenes, including the human carcinogen and common groundwater pollutant vinyl chloride (VC) as electron acceptors. Grape pomace microcosms and derived solid-free enrichment cultures were able to dechlorinate trichloroethene (TCE) to less chlorinated daughter products including ethene. 16S rRNA gene amplicon and qPCR analyses revealed a predominance of Dhgm sequences, but Dehalococcoides mccartyi (Dhc) biomarker genes were not detected. The enumeration of Dhgm 16S rRNA genes demonstrated VC-dependent growth, and 6.55±0.64 × 108 cells were measured per μmole of chloride released. Metagenome sequencing enabled the assembly of a Dhgm draft genome, and 52 putative reductive dehalogenase (RDase) genes were identified. Proteomic workflows identified a putative VC RDase with 49 and 56.1% amino acid similarity to the known VC RDases VcrA and BvcA, respectively. A survey of 1,173 groundwater samples collected from 111 chlorinated solvent-contaminated sites in the United States and Australia revealed that Dhgm 16S rRNA genes were frequently detected and outnumbered Dhc in 65% of the samples. Dhgm are likely greater contributors to reductive dechlorination of chlorinated solvents in contaminated aquifers than is currently recognized, and non-polluted environments represent sources of organohalide-respiring bacteria with novel RDase genes.\u003c/jats:p\u003e","long_citation_html":"Yang et al. (2017). Grape pomace compost harbors organohalide-respiring \u003ci\u003eDehalogenimonas\u003c/i\u003e species with novel reductive dehalogenase genes.\n\u003ci\u003eThe ISME Journal\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1038/ismej.2017.127\" target=\"_blank\"\u003eDOI: 10.1038/ismej.2017.127\u003c/a\u003e\n","created_at":"2026-10-22T10:55:03.636Z","updated_at":"2026-10-23T00:00:35.072Z","authors":[{"id":1569,"given":"Yi","family":"Yang","created_at":"2019-04-15T18:46:43.170Z","updated_at":"2019-04-15T18:46:43.170Z","url":"https://api.seqco.de/v1/authors/1569.json"},{"id":19887,"given":"Steven A","family":"Higgins","created_at":"2026-10-22T10:55:03.682Z","updated_at":"2026-10-22T10:55:03.682Z","url":"https://api.seqco.de/v1/authors/19887.json"},{"id":8109,"given":"Jun","family":"Yan","created_at":"2020-12-14T19:09:29.286Z","updated_at":"2020-12-14T19:09:29.286Z","url":"https://api.seqco.de/v1/authors/8109.json"},{"id":19888,"given":"Burcu","family":"Şimşir","created_at":"2026-10-22T10:55:03.701Z","updated_at":"2026-10-22T10:55:03.701Z","url":"https://api.seqco.de/v1/authors/19888.json"},{"id":19889,"given":"Karuna","family":"Chourey","created_at":"2026-10-22T10:55:03.714Z","updated_at":"2026-10-22T10:55:03.714Z","url":"https://api.seqco.de/v1/authors/19889.json"},{"id":19890,"given":"Ramsunder","family":"Iyer","created_at":"2026-10-22T10:55:03.725Z","updated_at":"2026-10-22T10:55:03.725Z","url":"https://api.seqco.de/v1/authors/19890.json"},{"id":19891,"given":"Robert L","family":"Hettich","created_at":"2026-10-22T10:55:03.737Z","updated_at":"2026-10-22T10:55:03.737Z","url":"https://api.seqco.de/v1/authors/19891.json"},{"id":19892,"given":"Brett","family":"Baldwin","created_at":"2026-10-22T10:55:03.751Z","updated_at":"2026-10-22T10:55:03.751Z","url":"https://api.seqco.de/v1/authors/19892.json"},{"id":19893,"given":"Dora M","family":"Ogles","created_at":"2026-10-22T10:55:03.763Z","updated_at":"2026-10-22T10:55:03.763Z","url":"https://api.seqco.de/v1/authors/19893.json"},{"id":19894,"given":"Frank E","family":"Löffler","created_at":"2026-10-22T10:55:03.775Z","updated_at":"2026-10-22T10:55:03.775Z","url":"https://api.seqco.de/v1/authors/19894.json"}],"names":[{"id":545,"name":"Candidatus Dehalogenimonas etheniformans","url":"https://api.seqco.de/v1/names/545.json","uri":"https://seqco.de/i:545"}],"subjects":[]}