{"response":{"status":"ok","message_type":"publication"},"id":4936,"citation":"Vieira et al., 2026, Applied and Environmental Microbiology","doi":"10.1128/aem.00981-26","url":"https://api.seqco.de/v1/publications/4936.json","link_ext":"https://doi.org/10.1128/aem.00981-26","title":"Chemical diversity analysis identifies inhibitory compounds in the secretomes of citrus-associated\n                    \u003ci\u003eBacillus\u003c/i\u003e\n                    spp. to the Huanglongbing pathogen, “\n                    \u003ci\u003eCandidatus\u003c/i\u003e\n                    Liberibacter asiaticus,” and citrus root pathogens","journal":"Applied and Environmental Microbiology","journal_loc":"","journal_date":"2026-10-22","pub_type":"journal-article","abstract":"\u003cjats:title\u003eABSTRACT\u003c/jats:title\u003e\n                  \u003cjats:sec\u003e\n                    \u003cjats:title/\u003e\n                    \u003cjats:p\u003e\n                      Huanglongbing (HLB) is a devastating and incurable citrus disease associated with the gram-negative, phloem-limited, and unculturable bacterium “\n                      \u003cjats:italic toggle=\"yes\"\u003eCandidatus\u003c/jats:italic\u003e\n                      Liberibacter asiaticus” (\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las), transmitted by the psyllid\n                      \u003cjats:italic toggle=\"yes\"\u003eDiaphorina citri\u003c/jats:italic\u003e\n                      . Our goal was to identify high-performing microbial candidates from a previously established citrus-associated microbial collection for the management of HLB and associated root pathogens. Candidate selection was based on bioactivity, genomic, and metabolomic profiles. Eighteen\n                      \u003cjats:italic toggle=\"yes\"\u003eBacillus\u003c/jats:italic\u003e\n                      isolates were screened for antagonistic activity against\n                      \u003cjats:italic toggle=\"yes\"\u003eLiberibacter crescens\u003c/jats:italic\u003e\n                      ,\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las, and citrus-associated\n                      \u003cjats:italic toggle=\"yes\"\u003ePhytophthora\u003c/jats:italic\u003e\n                      and\n                      \u003cjats:italic toggle=\"yes\"\u003eFusarium\u003c/jats:italic\u003e\n                      spp. Phylogenetic analysis grouped the isolates into four species groups:\n                      \u003cjats:italic toggle=\"yes\"\u003eBacillus velezensis\u003c/jats:italic\u003e\n                      ,\n                      \u003cjats:italic toggle=\"yes\"\u003eB. subtilis\u003c/jats:italic\u003e\n                      ,\n                      \u003cjats:italic toggle=\"yes\"\u003eB. pumilus\u003c/jats:italic\u003e\n                      , and\n                      \u003cjats:italic toggle=\"yes\"\u003eB. cereus\u003c/jats:italic\u003e\n                      . Among these,\n                      \u003cjats:italic toggle=\"yes\"\u003eB. velezensis\u003c/jats:italic\u003e\n                      exhibited the strongest and broadest inhibitory activity, followed by\n                      \u003cjats:italic toggle=\"yes\"\u003eB. subtilis\u003c/jats:italic\u003e\n                      . This enhanced antagonistic potential is likely associated with the greater number and diversity of biosynthetic gene clusters (BGCs) identified. Notably,\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las-inhibitory isolates included\n                      \u003cjats:italic toggle=\"yes\"\u003eB. velezensis\u003c/jats:italic\u003e\n                      (CB0021 and CB0909) and\n                      \u003cjats:italic toggle=\"yes\"\u003eB. altitudinis\u003c/jats:italic\u003e\n                      (CB0086). Bacilysin and surfactin BGCs were conserved across all\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las-inhibitory isolates, suggesting a potential role in\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las suppression. Accordingly, surfactins were detected in bioactive fractions of\n                      \u003cjats:italic toggle=\"yes\"\u003eB. velezensis\u003c/jats:italic\u003e\n                      (CB0027 and CB0909) and\n                      \u003cjats:italic toggle=\"yes\"\u003eB. subtilis\u003c/jats:italic\u003e\n                      (CB0893), supporting their likely contribution to bioactivity and inhibiting\n                      \u003cjats:italic toggle=\"yes\"\u003eL. crescens\u003c/jats:italic\u003e\n                      growth\n                      \u003cjats:italic toggle=\"yes\"\u003ein vitro\u003c/jats:italic\u003e\n                      , highlighting their potential as candidate compounds for\n                      \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                      Las management. Overall, this study underscores the importance of microbial discovery, screening, and characterization as an effective strategy for identifying sustainable solutions to manage HLB.\n                    \u003c/jats:p\u003e\n                    \u003cjats:sec\u003e\n                      \u003cjats:title\u003eIMPORTANCE\u003c/jats:title\u003e\n                      \u003cjats:p\u003e\n                        Huanglongbing (HLB) is a severe disease of citrus that has altered the citrus landscape worldwide and currently has no cure. It is caused by the bacterium “\n                        \u003cjats:italic toggle=\"yes\"\u003eCandidatus\u003c/jats:italic\u003e\n                        Liberibacter asiaticus” (\n                        \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                        Las) and transmitted by the psyllid\n                        \u003cjats:italic toggle=\"yes\"\u003eDiaphorina citri\u003c/jats:italic\u003e\n                        . Currently, the most effective control strategy for this disease relies on trunk injection of antibiotics oxytetracycline and streptomycin, and there is a need to find alternative solutions to complement or replace chemical control. Here, we aimed to select high-performing candidates from our previously established citrus-associated microbial collection to manage HLB and root-associated pathogens. We screened 18\n                        \u003cjats:italic toggle=\"yes\"\u003eBacillus\u003c/jats:italic\u003e\n                        isolates for antagonistic activity against\n                        \u003cjats:italic toggle=\"yes\"\u003eLiberibacter crescens\u003c/jats:italic\u003e\n                        ,\n                        \u003cjats:italic toggle=\"yes\"\u003eC\u003c/jats:italic\u003e\n                        Las, and citrus-associated\n                        \u003cjats:italic toggle=\"yes\"\u003ePhytophthora\u003c/jats:italic\u003e\n                        and\n                        \u003cjats:italic toggle=\"yes\"\u003eFusarium\u003c/jats:italic\u003e\n                        spp., and we generated their genomic and metabolomic profiles. Our research centers on selecting microbial candidates for their bioactivity and antimicrobial metabolite production, aiming to develop effective plant-protective bioinoculants for mitigation of HLB.\n                      \u003c/jats:p\u003e\n                    \u003c/jats:sec\u003e\n                  \u003c/jats:sec\u003e","long_citation_html":"Vieira et al. (2026). Chemical diversity analysis identifies inhibitory compounds in the secretomes of citrus-associated\n                    \u003ci\u003eBacillus\u003c/i\u003e\n                    spp. to the Huanglongbing pathogen, “\n                    \u003ci\u003eCandidatus\u003c/i\u003e\n                    Liberibacter asiaticus,” and citrus root pathogens.\n\u003ci\u003eApplied and Environmental Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1128/aem.00981-26\" target=\"_blank\"\u003eDOI: 10.1128/aem.00981-26\u003c/a\u003e\n","created_at":"2026-10-23T00:00:30.172Z","updated_at":"2026-10-24T00:00:18.006Z","authors":[{"id":7348,"given":"Flavia Campos","family":"Vieira","created_at":"2020-04-05T06:30:56.936Z","updated_at":"2020-04-05T06:30:56.936Z","url":"https://api.seqco.de/v1/authors/7348.json"},{"id":7939,"given":"Manikandan","family":"Ramasamy","created_at":"2020-12-14T19:08:26.652Z","updated_at":"2020-12-14T19:08:26.652Z","url":"https://api.seqco.de/v1/authors/7939.json"},{"id":10796,"given":"Kranthi","family":"Mandadi","created_at":"2022-10-06T22:00:26.638Z","updated_at":"2022-10-06T22:00:26.638Z","url":"https://api.seqco.de/v1/authors/10796.json"},{"id":19932,"given":"Mariana Bossi","family":"Esteves","created_at":"2026-10-23T00:00:30.568Z","updated_at":"2026-10-23T00:00:30.568Z","url":"https://api.seqco.de/v1/authors/19932.json"},{"id":16382,"given":"Amancio","family":"de Souza","created_at":"2025-08-01T22:00:33.605Z","updated_at":"2025-08-01T22:00:33.605Z","url":"https://api.seqco.de/v1/authors/16382.json"},{"id":19933,"given":"Macie","family":"Allen","created_at":"2026-10-23T00:00:30.845Z","updated_at":"2026-10-23T00:00:30.845Z","url":"https://api.seqco.de/v1/authors/19933.json"},{"id":19713,"given":"Carolina","family":"Ruiz Rivera","created_at":"2026-10-14T00:00:23.667Z","updated_at":"2026-10-14T00:00:23.667Z","url":"https://api.seqco.de/v1/authors/19713.json"},{"id":16387,"given":"Christopher","family":"Yang","created_at":"2025-08-01T22:00:33.649Z","updated_at":"2025-08-01T22:00:33.649Z","url":"https://api.seqco.de/v1/authors/16387.json"},{"id":7256,"given":"Katherine N.","family":"Maloney","created_at":"2020-02-23T07:30:43.989Z","updated_at":"2020-02-23T07:30:43.989Z","url":"https://api.seqco.de/v1/authors/7256.json"},{"id":7258,"given":"M. Caroline","family":"Roper","created_at":"2020-02-23T07:30:44.455Z","updated_at":"2020-02-23T07:30:44.455Z","url":"https://api.seqco.de/v1/authors/7258.json"}],"names":[{"id":1,"name":"Liberibacter asiaticus","url":"https://api.seqco.de/v1/names/1.json","uri":"https://seqco.de/i:1"}],"subjects":[]}