{"response":{"status":"ok","message_type":"publication"},"id":4944,"citation":"Hatzenpichler et al., 2008, Proceedings of the National Academy of Sciences","doi":"10.1073/pnas.0708857105","url":"https://api.seqco.de/v1/publications/4944.json","link_ext":"https://doi.org/10.1073/pnas.0708857105","title":"A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring","journal":"Proceedings of the National Academy of Sciences","journal_loc":"105 (6)","journal_date":"2008-02-12","pub_type":"journal-article","abstract":"\u003cjats:p\u003e\n                    The recent discovery of ammonia-oxidizing archaea (AOA) dramatically changed our perception of the diversity and evolutionary history of microbes involved in nitrification. In this study, a moderately thermophilic (46°C) ammonia-oxidizing enrichment culture, which had been seeded with biomass from a hot spring, was screened for ammonia oxidizers. Although gene sequences for crenarchaeotal 16S rRNA and two subunits of the ammonia monooxygenase (\n                    \u003cjats:italic\u003eamoA\u003c/jats:italic\u003e\n                    and\n                    \u003cjats:italic\u003eamoB\u003c/jats:italic\u003e\n                    ) were detected via PCR, no hints for known ammonia-oxidizing bacteria were obtained. Comparative sequence analyses of these gene fragments demonstrated the presence of a single operational taxonomic unit and thus enabled the assignment of the\n                    \u003cjats:italic\u003eamoA\u003c/jats:italic\u003e\n                    and\n                    \u003cjats:italic\u003eamoB\u003c/jats:italic\u003e\n                    sequences to the respective 16S rRNA phylotype, which belongs to the widely distributed group I.1b (soil group) of the\n                    \u003cjats:italic\u003eCrenarchaeota\u003c/jats:italic\u003e\n                    . Catalyzed reporter deposition (CARD)–FISH combined with microautoradiography (MAR) demonstrated metabolic activity of this archaeon in the presence of ammonium. This finding was corroborated by the detection of\n                    \u003cjats:italic\u003eamoA\u003c/jats:italic\u003e\n                    gene transcripts in the enrichment. CARD-FISH/MAR showed that the moderately thermophilic AOA is highly active at 0.14 and 0.79 mM ammonium and is partially inhibited by a concentration of 3.08 mM. The enriched AOA, which is provisionally classified as “\n                    \u003cjats:italic\u003eCandidatus\u003c/jats:italic\u003e\n                    Nitrososphaera gargensis,” is the first described thermophilic ammonia oxidizer and the first member of the crenarchaeotal group I.1b for which ammonium oxidation has been verified on a cellular level. Its preference for thermophilic conditions reinvigorates the debate on the thermophilic ancestry of AOA.\n                  \u003c/jats:p\u003e","long_citation_html":"Hatzenpichler et al. (2008). A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring.\n\u003ci\u003eProceedings of the National Academy of Sciences\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1073/pnas.0708857105\" target=\"_blank\"\u003eDOI: 10.1073/pnas.0708857105\u003c/a\u003e\n","created_at":"2026-10-23T09:04:04.573Z","updated_at":"2026-10-24T00:00:18.981Z","authors":[{"id":10497,"given":"Roland","family":"Hatzenpichler","created_at":"2022-07-03T17:09:46.563Z","updated_at":"2022-07-03T17:09:46.563Z","url":"https://api.seqco.de/v1/authors/10497.json"},{"id":19267,"given":"Elena V.","family":"Lebedeva","created_at":"2026-08-27T11:29:31.831Z","updated_at":"2026-08-27T11:29:31.831Z","url":"https://api.seqco.de/v1/authors/19267.json"},{"id":3461,"given":"Eva","family":"Spieck","created_at":"2019-04-15T18:47:55.481Z","updated_at":"2019-04-15T18:47:55.481Z","url":"https://api.seqco.de/v1/authors/3461.json"},{"id":9702,"given":"Kilian","family":"Stoecker","created_at":"2021-12-15T17:21:38.202Z","updated_at":"2021-12-15T17:21:38.202Z","url":"https://api.seqco.de/v1/authors/9702.json"},{"id":10430,"given":"Andreas","family":"Richter","created_at":"2022-06-15T14:50:40.450Z","updated_at":"2022-06-15T14:50:40.450Z","url":"https://api.seqco.de/v1/authors/10430.json"},{"id":3087,"given":"Holger","family":"Daims","created_at":"2019-04-15T18:47:43.308Z","updated_at":"2019-04-15T18:47:43.308Z","url":"https://api.seqco.de/v1/authors/3087.json"},{"id":324,"given":"Michael","family":"Wagner","created_at":"2019-04-15T18:46:01.846Z","updated_at":"2019-04-15T18:46:01.846Z","url":"https://api.seqco.de/v1/authors/324.json"}],"names":[{"id":191,"name":"Candidatus Nitrososphaera gargensis","url":"https://api.seqco.de/v1/names/191.json","uri":"https://seqco.de/i:191"}],"subjects":[]}