{"response":{"status":"ok","message_type":"publication"},"id":4965,"citation":"Anstead, Chilton, 2013, Applied and Environmental Microbiology","doi":"10.1128/aem.02286-13","url":"https://api.seqco.de/v1/publications/4965.json","link_ext":"https://doi.org/10.1128/aem.02286-13","title":"A Novel Rickettsia Species Detected in Vole Ticks (Ixodes angustus) from Western Canada","journal":"Applied and Environmental Microbiology","journal_loc":"79 (24)","journal_date":"2013-12-15","pub_type":"journal-article","abstract":"\u003cjats:title\u003eABSTRACT\u003c/jats:title\u003e\u003cjats:p\u003eThe genomic DNA of ixodid ticks from western Canada was tested by PCR for the presence of\u003cjats:named-content content-type=\"genus-species\"\u003eRickettsia\u003c/jats:named-content\u003e. No rickettsiae were detected in\u003cjats:named-content content-type=\"genus-species\"\u003eIxodes sculptus\u003c/jats:named-content\u003e, whereas 18% of the\u003cjats:named-content content-type=\"genus-species\"\u003eI. angustus\u003c/jats:named-content\u003eand 42% of the\u003cjats:named-content content-type=\"genus-species\"\u003eDermacentor andersoni\u003c/jats:named-content\u003eorganisms examined were PCR positive for\u003cjats:named-content content-type=\"genus-species\"\u003eRickettsia\u003c/jats:named-content\u003e. The rickettsiae from each tick species were characterized genetically using multiple genes. Rickettsiae within the\u003cjats:named-content content-type=\"genus-species\"\u003eD. andersoni\u003c/jats:named-content\u003eorganisms had sequences at four genes that matched those of\u003cjats:named-content content-type=\"genus-species\"\u003eR. peacockii\u003c/jats:named-content\u003e. In contrast, the\u003cjats:named-content content-type=\"genus-species\"\u003eRickettsia\u003c/jats:named-content\u003epresent within the larvae, nymphs, and adults of\u003cjats:named-content content-type=\"genus-species\"\u003eI. angustus\u003c/jats:named-content\u003ehad novel DNA sequences at four of the genes characterized compared to the sequences available from GenBank for all recognized species of\u003cjats:named-content content-type=\"genus-species\"\u003eRickettsia\u003c/jats:named-content\u003eand all other putative species within the genus. Phylogenetic analyses of the sequence data revealed that the rickettsiae in\u003cjats:named-content content-type=\"genus-species\"\u003eI. angustus\u003c/jats:named-content\u003edo not belong to the spotted fever, transitional, or typhus groups of rickettsiae but are most closely related to “\u003cjats:named-content content-type=\"genus-species\"\u003eCandidatus\u003c/jats:named-content\u003eRickettsia kingi” and belong to a clade that also includes\u003cjats:named-content content-type=\"genus-species\"\u003eR. canadensis\u003c/jats:named-content\u003e, “\u003cjats:named-content content-type=\"genus-species\"\u003eCandidatus\u003c/jats:named-content\u003eRickettsia tarasevichiae,” and “\u003cjats:named-content content-type=\"genus-species\"\u003eCandidatus\u003c/jats:named-content\u003eRickettsia monteiroi.”\u003c/jats:p\u003e","long_citation_html":"Anstead, Chilton (2013). A Novel Rickettsia Species Detected in Vole Ticks (Ixodes angustus) from Western Canada.\n\u003ci\u003eApplied and Environmental Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.1128/aem.02286-13\" target=\"_blank\"\u003eDOI: 10.1128/aem.02286-13\u003c/a\u003e\n","created_at":"2026-11-08T09:54:25.164Z","updated_at":"2026-11-09T00:00:20.784Z","authors":[{"id":20021,"given":"Clare A.","family":"Anstead","created_at":"2026-11-08T09:54:25.172Z","updated_at":"2026-11-08T09:54:25.172Z","url":"https://api.seqco.de/v1/authors/20021.json"},{"id":3054,"given":"Neil B.","family":"Chilton","created_at":"2019-04-15T18:47:42.252Z","updated_at":"2019-04-15T18:47:42.252Z","url":"https://api.seqco.de/v1/authors/3054.json"}],"names":[{"id":57180,"name":"Rickettsia angusta","url":"https://api.seqco.de/v1/names/57180.json","uri":"https://seqco.de/i:57180"}],"subjects":[]}