{"response":{"status":"ok","message_type":"publication"},"id":4992,"citation":"Liu et al., 2026, Frontiers in Microbiology","doi":"10.3389/fmicb.2025.1683098","url":"https://api.seqco.de/v1/publications/4992.json","link_ext":"https://doi.org/10.3389/fmicb.2025.1683098","title":"Reporting two novel Kluyvera species, Kluyvera huaxiensis and Kluyvera chengduensis, isolated from human sputa","journal":"Frontiers in Microbiology","journal_loc":"16","journal_date":"2026-01-05","pub_type":"journal-article","abstract":"\u003cjats:sec\u003e\n                    \u003cjats:title\u003eIntroduction\u003c/jats:title\u003e\n                    \u003cjats:p\u003e\n                      Two Gram-stain negative, facultatively anaerobic, rod-shaped, motile, and non-spore forming bacterial strains, 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      , were isolated from sputum samples obtained from different patients.\n                    \u003c/jats:p\u003e\n                  \u003c/jats:sec\u003e\n                  \u003cjats:sec\u003e\n                    \u003cjats:title\u003eMethods\u003c/jats:title\u003e\n                    \u003cjats:p\u003e\n                      Growth of strain 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      occurred at 8–42 °C (optimum 28–37 °C), pH 5.0–9.0 (optimum 6.0–8.0) and NaCl concentrations of 0–5% (w/v) (optimum 0–4%). Comparison of 16S rRNA gene sequences showed that strain 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      exhibited high similarity to\n                      \u003cjats:italic\u003eKluyvera intermedia\u003c/jats:italic\u003e\n                      NBRC 102594\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      (99.48%) and\n                      \u003cjats:italic\u003eKluyvera georgiana\u003c/jats:italic\u003e\n                      ATCC 51603\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      (98.68%), respectively.\n                    \u003c/jats:p\u003e\n                  \u003c/jats:sec\u003e\n                  \u003cjats:sec\u003e\n                    \u003cjats:title\u003eResults\u003c/jats:title\u003e\n                    \u003cjats:p\u003e\n                      Phylogenomic tree of species from family\n                      \u003cjats:italic\u003eEnterobacteriaceae\u003c/jats:italic\u003e\n                      revealed that strain 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      formed distinct branches within the\n                      \u003cjats:italic\u003eKluyvera\u003c/jats:italic\u003e\n                      clade. The digital DNA–DNA hybridization between 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      , 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      , and all type strains of\n                      \u003cjats:italic\u003eKluyvera\u003c/jats:italic\u003e\n                      species were ≤39.5% and ≤54.7%, respectively, and the corresponding average nucleotide identity were ≤90.56% and ≤93.85%, both below the species delineation threshold. The two strains differ phenotypically from other\n                      \u003cjats:italic\u003eKluyvera\u003c/jats:italic\u003e\n                      species by their lipase and arginine dihydrolase utilization and from each other by ᴅ-arabitol utilization. The predominant fatty acids identified in both strains were C\n                      \u003cjats:sub\u003e16:0\u003c/jats:sub\u003e\n                      , summed feature 3 (C\n                      \u003cjats:sub\u003e16:1\u003c/jats:sub\u003e\n                      \u003cjats:italic\u003eω7c\u003c/jats:italic\u003e\n                      /C\n                      \u003cjats:sub\u003e16:1\u003c/jats:sub\u003e\n                      \u003cjats:italic\u003eω6c\u003c/jats:italic\u003e\n                      ), summed feature 8 (C\n                      \u003cjats:sub\u003e18:1\u003c/jats:sub\u003e\n                      \u003cjats:italic\u003eω7c\u003c/jats:italic\u003e\n                      ) and C\n                      \u003cjats:sub\u003e17:0\u003c/jats:sub\u003e\n                      cyclo.\n                    \u003c/jats:p\u003e\n                  \u003c/jats:sec\u003e\n                  \u003cjats:sec\u003e\n                    \u003cjats:title\u003eDiscussion\u003c/jats:title\u003e\n                    \u003cjats:p\u003e\n                      Based on the phenotypic, chemotaxonomic, phylogenetic and genomic evidence, strains 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      represent two novel species of the genus\n                      \u003cjats:italic\u003eKluyvera\u003c/jats:italic\u003e\n                      , for which the names\n                      \u003cjats:italic\u003eKluyvera huaxiensis\u003c/jats:italic\u003e\n                      sp. nov. and\n                      \u003cjats:italic\u003eKluyvera chengduensis\u003c/jats:italic\u003e\n                      sp. nov. are proposed, with type strains 142053\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      (= GDMCC 1.4845\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      = JCM 37413\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      ) and 142359\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      (= GDMCC 1.5094\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      = JCM 37737\n                      \u003cjats:sup\u003eT\u003c/jats:sup\u003e\n                      ), respectively.\n                    \u003c/jats:p\u003e\n                  \u003c/jats:sec\u003e","long_citation_html":"Liu et al. (2026). Reporting two novel Kluyvera species, Kluyvera huaxiensis and Kluyvera chengduensis, isolated from human sputa.\n\u003ci\u003eFrontiers in Microbiology\u003c/i\u003e.\n\u003ca href=\"https://doi.org/10.3389/fmicb.2025.1683098\" target=\"_blank\"\u003eDOI: 10.3389/fmicb.2025.1683098\u003c/a\u003e\n","created_at":"2026-11-10T00:47:49.557Z","updated_at":"2026-11-10T00:47:49.557Z","authors":[{"id":20140,"given":"Lina","family":"Liu","created_at":"2026-11-10T00:47:49.566Z","updated_at":"2026-11-10T00:47:49.566Z","url":"https://api.seqco.de/v1/authors/20140.json"},{"id":14762,"given":"Yu","family":"Feng","created_at":"2024-08-22T02:41:02.936Z","updated_at":"2024-08-22T02:41:02.936Z","url":"https://api.seqco.de/v1/authors/14762.json"},{"id":14768,"given":"Hongxia","family":"Wen","created_at":"2024-08-22T02:41:03.034Z","updated_at":"2024-08-22T02:41:03.034Z","url":"https://api.seqco.de/v1/authors/14768.json"},{"id":14770,"given":"Zhiyong","family":"Zong","created_at":"2024-08-22T02:41:03.071Z","updated_at":"2024-08-22T02:41:03.071Z","url":"https://api.seqco.de/v1/authors/14770.json"}],"names":[{"id":49036,"name":"Kluyvera chengduensis","url":"https://api.seqco.de/v1/names/49036.json","uri":"https://seqco.de/i:49036"},{"id":46779,"name":"Kluyvera huaxiensis","url":"https://api.seqco.de/v1/names/46779.json","uri":"https://seqco.de/i:46779"}],"subjects":[]}