{"response":{"status":"ok","message_type":"publication"},"id":684,"citation":"Jones et al., 2015, Applied and Environmental Microbiology","doi":"10.1128/aem.00039-15","url":"https://api.seqco.de/v1/publications/684.json","link_ext":"https://doi.org/10.1128/aem.00039-15","title":"Metatranscriptomic Analysis of Diminutive Thiomargarita-Like Bacteria (“Candidatus Thiopilula” spp.) from Abyssal Cold Seeps of the Barbados Accretionary Prism","journal":"Applied and Environmental Microbiology","journal_loc":"81 (9)","journal_date":"2015-05-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eABSTRACT\u003c/jats:title\u003e\n          \u003cjats:p\u003e\n            Large sulfur-oxidizing bacteria in the family\n            \u003cjats:named-content content-type=\"genus-species\"\u003eBeggiatoaceae\u003c/jats:named-content\u003e\n            are important players in the global sulfur cycle. This group contains members of the well-known genera\n            \u003cjats:named-content content-type=\"genus-species\"\u003eBeggiatoa\u003c/jats:named-content\u003e\n            ,\n            \u003cjats:named-content content-type=\"genus-species\"\u003eThioploca\u003c/jats:named-content\u003e\n            , and\n            \u003cjats:named-content content-type=\"genus-species\"\u003eThiomargarita\u003c/jats:named-content\u003e\n            but also recently identified and relatively unknown candidate taxa, including “\n            \u003cjats:named-content content-type=\"genus-species\"\u003eCandidatus\u003c/jats:named-content\u003e\n            Thiopilula” spp. and “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Thiophysa” spp. We discovered a population of “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Thiopilula” spp. colonizing cold seeps near Barbados at a ∼4.7-km water depth. The Barbados population consists of spherical cells that are morphologically similar to\n            \u003cjats:named-content content-type=\"genus-species\"\u003eThiomargarita\u003c/jats:named-content\u003e\n            spp., with elemental sulfur inclusions and a central vacuole, but have much smaller cell diameters (5 to 40 μm). Metatranscriptomic analysis revealed that when exposed to anoxic sulfidic conditions, Barbados “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Thiopilula” organisms expressed genes for the oxidation of elemental sulfur and the reduction of nitrogenous compounds, consistent with their vacuolated morphology and intracellular sulfur storage capability. Metatranscriptomic analysis further revealed that anaerobic methane-oxidizing and sulfate-reducing organisms were active in the sediment, which likely provided reduced sulfur substrates for “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Thiopilula” and other sulfur-oxidizing microorganisms in the community. The novel observations of “\n            \u003cjats:italic\u003eCa\u003c/jats:italic\u003e\n            . Thiopilula” and associated organisms reported here expand our knowledge of the globally distributed and ecologically successful\n            \u003cjats:named-content content-type=\"genus-species\"\u003eBeggiatoaceae\u003c/jats:named-content\u003e\n            group and thus offer insight into the composition and ecology of deep cold seep microbial communities.\n          \u003c/jats:p\u003e","long_citation_html":"Jones et al. (2015). Metatranscriptomic Analysis of Diminutive Thiomargarita-Like Bacteria (“Candidatus Thiopilula” spp.) from Abyssal Cold Seeps of the Barbados Accretionary Prism. \n\u003ci\u003eApplied and Environmental Microbiology\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1128/aem.00039-15\" target=\"_blank\"\u003eDOI:10.1128/aem.00039-15\u003c/a\u003e\n","created_at":"2019-04-15T18:47:30.517Z","updated_at":"2025-11-06T11:35:29.718Z","authors":[{"id":2704,"given":"Daniel S.","family":"Jones","created_at":"2019-04-15T18:47:30.523Z","updated_at":"2019-04-15T18:47:30.523Z","url":"https://api.seqco.de/v1/authors/2704.json"},{"id":2705,"given":"Beverly E.","family":"Flood","created_at":"2019-04-15T18:47:30.530Z","updated_at":"2019-04-15T18:47:30.530Z","url":"https://api.seqco.de/v1/authors/2705.json"},{"id":2706,"given":"Jake V.","family":"Bailey","created_at":"2019-04-15T18:47:30.539Z","updated_at":"2019-04-15T18:47:30.539Z","url":"https://api.seqco.de/v1/authors/2706.json"}],"names":[{"id":32,"name":"Candidatus Thiophysa","url":"https://api.seqco.de/v1/names/32.json","uri":"https://seqco.de/i:32"},{"id":33,"name":"Candidatus Thiopilula","url":"https://api.seqco.de/v1/names/33.json","uri":"https://seqco.de/i:33"}],"subjects":[{"id":23,"name":"Applied Microbiology and Biotechnology","url":"https://api.seqco.de/v1/subjects/23.json"},{"id":4,"name":"Biotechnology","url":"https://api.seqco.de/v1/subjects/4.json"},{"id":6,"name":"Ecology","url":"https://api.seqco.de/v1/subjects/6.json"},{"id":22,"name":"Food Science","url":"https://api.seqco.de/v1/subjects/22.json"}]}