{"response":{"status":"ok","message_type":"publication"},"id":848,"citation":"García‐Amado et al., 2007, Helicobacter","doi":"10.1111/j.1523-5378.2007.00526.x","url":"https://api.seqco.de/v1/publications/848.json","link_ext":"https://doi.org/10.1111/j.1523-5378.2007.00526.x","title":"Non‐\u003ci\u003epylori \u003c/i\u003eHelicobacteraceae in the Upper Digestive Tract of Asymptomatic Venezuelan Subjects: Detection of\u003ci\u003e Helicobacter cetorum‐\u003c/i\u003elike and Candidatus \u003ci\u003eWolinella africanus\u003c/i\u003e‐like DNA","journal":"Helicobacter","journal_loc":"12 (5)","journal_date":"2007-10-01","pub_type":"journal-article","abstract":"\u003cjats:title\u003eAbstract\u003c/jats:title\u003e\u003cjats:p\u003e\u003cjats:bold\u003eBackground: \u003c/jats:bold\u003e The spectrum of human non‐\u003cjats:italic\u003epylori Helicobacter\u003c/jats:italic\u003e infections is expanding, with species such as \u003cjats:italic\u003eH. heilmannii\u003c/jats:italic\u003e and \u003cjats:italic\u003eH. felis\u003c/jats:italic\u003e occasionally being associated with gastritis. However, the existence of non‐\u003cjats:italic\u003epylori Helicobacter\u003c/jats:italic\u003e colonization in asymptomatic subjects has not been evaluated. The aim of this study was to investigate whether \u003cjats:italic\u003eHelicobacter\u003c/jats:italic\u003e species other than \u003cjats:italic\u003epylori \u003c/jats:italic\u003eare present in the upper digestive tract of asymptomatic human subjects.\u003c/jats:p\u003e\u003cjats:p\u003e\u003cjats:bold\u003eMaterials and methods: \u003c/jats:bold\u003e A Helicobacteraceae‐specific semi‐nested polymerase chain reaction (PCR) assay was used to detect \u003cjats:italic\u003eHelicobacter‐\u003c/jats:italic\u003elike organisms in the upper digestive tract of 91 Venezuelan volunteers (aged 18–68 years, 41 females, 50 males). Species were identified by denaturing gradient gel electrophoresis analysis and sequencing of the PCR products.\u003c/jats:p\u003e\u003cjats:p\u003e\u003cjats:bold\u003eResults: \u003c/jats:bold\u003e We detected DNA sharing 99–100% sequence identity in over 300–400 bp with the 16S rRNA genes of \u003cjats:italic\u003eH. pylori\u003c/jats:italic\u003e, \u003cjats:italic\u003eH. cetorum\u003c/jats:italic\u003e, and Candidatus \u003cjats:italic\u003eWolinella africanus\u003c/jats:italic\u003e in 76%, 16%, and 15% of the subjects, respectively. Multiple colonization was documented in 10% of the subjects: \u003cjats:italic\u003eH. cetorum\u003c/jats:italic\u003e and Candidatus \u003cjats:italic\u003eW. africanus\u003c/jats:italic\u003e (4%), \u003cjats:italic\u003eH. pylori \u003c/jats:italic\u003eand Candidatus \u003cjats:italic\u003eW. africanus\u003c/jats:italic\u003e (4%), and \u003cjats:italic\u003eH. pylori\u003c/jats:italic\u003e and \u003cjats:italic\u003eH. cetorum\u003c/jats:italic\u003e (2%).\u003c/jats:p\u003e\u003cjats:p\u003e\u003cjats:bold\u003eConclusions: \u003c/jats:bold\u003e Our results suggest that non‐\u003cjats:italic\u003epylori \u003c/jats:italic\u003eHelicobacteraceae colonization is relatively common in the Venezuelan asymptomatic population. This is the first report documenting the presence of \u003cjats:italic\u003eH. cetorum\u003c/jats:italic\u003e DNA in the human upper digestive tract, and the second report of the recently discovered Candidatus \u003cjats:italic\u003eW. africanus\u003c/jats:italic\u003e.\u003c/jats:p\u003e","long_citation_html":"García‐Amado et al. (2007). Non‐\u003ci\u003epylori \u003c/i\u003eHelicobacteraceae in the Upper Digestive Tract of Asymptomatic Venezuelan Subjects: Detection of\u003ci\u003e Helicobacter cetorum‐\u003c/i\u003elike and Candidatus \u003ci\u003eWolinella africanus\u003c/i\u003e‐like DNA. \n\u003ci\u003eHelicobacter\u003c/i\u003e. \u003ca href=\"https://doi.org/10.1111/j.1523-5378.2007.00526.x\" target=\"_blank\"\u003eDOI:10.1111/j.1523-5378.2007.00526.x\u003c/a\u003e\n","created_at":"2019-04-15T18:47:50.248Z","updated_at":"2025-11-06T11:37:29.641Z","authors":[{"id":17145,"given":"M. Alexandra","family":"García‐Amado","created_at":"2025-11-06T11:37:29.652Z","updated_at":"2025-11-06T11:37:29.652Z","url":"https://api.seqco.de/v1/authors/17145.json"},{"id":17146,"given":"Waleed Abu","family":"Al‐Soud","created_at":"2025-11-06T11:37:29.664Z","updated_at":"2025-11-06T11:37:29.664Z","url":"https://api.seqco.de/v1/authors/17146.json"},{"id":17147,"given":"Pedro","family":"Borges‐Landaéz","created_at":"2025-11-06T11:37:29.675Z","updated_at":"2025-11-06T11:37:29.675Z","url":"https://api.seqco.de/v1/authors/17147.json"},{"id":3338,"given":"Monica","family":"Contreras","created_at":"2019-04-15T18:47:50.290Z","updated_at":"2019-04-15T18:47:50.290Z","url":"https://api.seqco.de/v1/authors/3338.json"},{"id":3339,"given":"Samandhy","family":"Cedeño","created_at":"2019-04-15T18:47:50.298Z","updated_at":"2019-04-15T18:47:50.298Z","url":"https://api.seqco.de/v1/authors/3339.json"},{"id":17148,"given":"Estalina","family":"Baéz‐Ramírez","created_at":"2025-11-06T11:37:29.698Z","updated_at":"2025-11-06T11:37:29.698Z","url":"https://api.seqco.de/v1/authors/17148.json"},{"id":17149,"given":"M. Gloria","family":"Domínguez‐Bello","created_at":"2025-11-06T11:37:29.708Z","updated_at":"2025-11-06T11:37:29.708Z","url":"https://api.seqco.de/v1/authors/17149.json"},{"id":3342,"given":"Torkel","family":"Wadström","created_at":"2019-04-15T18:47:50.327Z","updated_at":"2019-04-15T18:47:50.327Z","url":"https://api.seqco.de/v1/authors/3342.json"},{"id":3343,"given":"Pulchérie","family":"Gueneau","created_at":"2019-04-15T18:47:50.337Z","updated_at":"2019-04-15T18:47:50.337Z","url":"https://api.seqco.de/v1/authors/3343.json"}],"names":[{"id":451,"name":"Candidatus Wolinella africanus","url":"https://api.seqco.de/v1/names/451.json","uri":"https://seqco.de/i:451"}],"subjects":[{"id":32,"name":"Gastroenterology","url":"https://api.seqco.de/v1/subjects/32.json"},{"id":11,"name":"General Medicine","url":"https://api.seqco.de/v1/subjects/11.json"},{"id":17,"name":"Infectious Diseases","url":"https://api.seqco.de/v1/subjects/17.json"}]}